The present invention is directed to methods and kits for isolation of bacteria from biological samples. In such methods antibodies specific for eukaryotic cells, which are deficient in a bacteria-binding Fc-terminus, are used for separating eukaryotic cells from biological samples.Juergen Klepp...
Nucleic-acid (NA) based techniques offer a rapid and highly sensitive option for detecting pathogenic bacteria directly from specimens when compared with culture techniques [2], [3], [4], [5], [6]. However, besides inherent limitations of primer-based PCR methods such as the presence of ...
Cell isolation—also referred to ascell separationor cell sorting—is the process of isolating one cell population from other cells in a heterogeneous biological sample. Targeted cells are identified, isolated, and then separated according to their type. What are some example uses of cell isolation?
申请(专利权)人: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM 发明人:JE Becvar,Laura Becvar 被引量: 22 摘要: Methods for the isolation and identification of a toxicant in a sample are disclosed. Luminescent biological agents (i.e., bacteria) having sensitivity to a toxicant or an ...
To evaluate which combination of decontamination method and medium is most reliable when examining acidic, organic forest soils for mycobacteria, three decontamination methods and five media supplemented with cycloheximide were compared. Before decontamination, the samples were incubated at 37°C for 5 h...
Isolation, purification, immobilization of oxalate oxidase and its clinical applications. Oxalate oxidase known to catalyse the aerobic oxidation of oxalic acid into CO2 and H2O2, has been found in bacteria, fungi, mosses and some higher plants. So far, a membrane bound oxalate oxidase from Pseudom...
The bacteria were first dispersed and separated from soil pa... William,E.,Holben,... - 《Applied & Environmental Microbiology》 被引量: 1337发表: 1988年 The extraction and purification of microbial DNA from sediments A new method for the isolation of intracellular and extacellular DNA from a...
1.Pick a colony of bacteria harboring the plasmid or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100μg/ml)and incubate at 37deg C 8-10 hours with shaking at 250 rpm.Transfer the...
The new strains have the capability to use PCBs as a source of sole carbon and harbor 2,3-dihydroxybiphenyl dioxygenase (DHBDO) which could be used as molecular marker for detection PCBs-degrading bacteria in the PCBs contaminated sites. This finding may enhance the PCBs bioremediation by ...
of bacteria are unculturable or require very specific growth conditions3. In recent years, metagenomics, e.g., sequencing of total DNA isolated directly from collected samples allowed to estimate the real diversity and abundance of environmental microorganisms bypassing the isolation and cultivation steps...