metaphase spreads:中期利差 metaphase中期
中期染色体涂片
Drop the cells onto pre-cleaned slide or treated slide above, rinse with fixative (optional), dry on wet tower papers, and observe for metaphase spreads with a phase-constrast microscope.
This method is highly labour intensive and cannot be performed outside the preimplantation genetic diagnosis (PGD) laboratory, so to overcome these limitations, a method was developed for obtaining metaphase spreads from single biopsied blastomeres using different chemicals. The substances tested were ...
In the "direct" technique, presented here in an Alternate Protocol, Langhans of the cytotrophoblast, actively dividing in first-trimester villi, are synchronized and arrested in after a short incubation period, and spreads are prepared. Chorionic villi are composed of an outer layer of ...
Automatic identification of metaphase spreads and nuclei using neural networks. Med Biol Eng Comput. 2001; 39 :391–396.F. A. Cosio, L. Vega, et al., "Automatic identification of metaphase spreads and nuclei using neural networks", Medical & Biological Engineering & Computing, 39(3), 391-...
Carayannopoulos, GL, Patrick, EA (1976) An algorithm for segmentation of metaphase speads. Pattern Recognition, Vol 161: pp. 151PATTERN RECOGNITION, Jahrgang 8, Nr. 3, Juli 1976, Oxford, GB, G. CARAYANNOPOULOS et al.: " An algorithm for segmentation of metaphase spreads ", Seiten 151-...
This protocol outlines the generation of metaphase spreads of a single oocyte at either the immature metaphase I (MI) or mature metaphase II (MII) stage. This protocol utilizes formaldehyde fixation and therefore allows for further immunofluorescence analysis. These spreads can also be used to identi...
Preparation of Metaphase Spreads from Chorionic Villus Samplesdoi:10.1002/0471142905.hg0803s00Laird JacksonJefferson Medical College Philadelphia PennsylvaniaLongina M. GibasJefferson Medical College Philadelphia PennsylvaniaMarie A. BarrJefferson Medical College Philadelphia Pennsylvania...
Uhlmann V,,Prasad M,Silva I, et al.Improved in situ detection method for telomeric tandem repeats in metaphase spreads and interphase nuclei[ J].J C lin Pathol: Mol Pathol. 2000Uhlmann V et al. Improved in situ detection method for telomeric tandem repeats in metaphase spreads and interphase...