Electron microscopy and atomic force microscopy studies of chromatin and metaphase chromosome structure 6 Metaphase chromosomes in organic media and at very low cation concentration In the typical metaphase cell
Metaphase chromosome spreads after C-banding.José, Carlos PansonatoAlvesÉrica, Alves SerranoRicardo, UtsunomiaJuan, Pedro M. CamachoGuilherme, José da Costa SilvaMarcelo, Ricardo VicariRoberto, Ferreira ArtoniClaudio, OliveiraFausto, Foresti
aI NEED YOU I WAND YOU I FUCK YOU I NEED YOU I WAND YOU I FUCK YOU [translate] a人们很关心气候变化 正在翻译,请等待... [translate] a匝间试验设备 正在翻译,请等待... [translate] aPreparation of Metaphase Chromosome Spreads 分裂中期染色体传播的准备 [translate] ...
however, very time consuming since they are selected manually. The selection of suitable metaphase chromosome spreads thus represents a major bottleneck for conventional cytogenetic
Methods Synchronized cultured cells were fixed with methanol/acetic acid to prepare chromosome spreads in teflon-coated diagnostic well-slides. Apart from the slide format and the chromosome spreading everything was done essentially according to standard protocols. Hybridization targets were detected in ...
The frequency of SCE can thus be enumerated in metaphase chromosome spreads prepared from cells toward the end of the second mitosis following BrdU treatment. Sister Chromatid Exchange and Genomic Instability in Soft Tissue Sarcomas: Potential Implications for Response to DNA-Damaging Treatments More spe...
18. Air dry the chromosome slide, check for chromosome spreading and cytoplasm debris in a phase contrast lab microscope, adjust volume of fixative so that the density of nuclei/metaphases is appropriate 19. If the conditons are favorable, prepare a batch of metaphases spreads 20. Keep slide...
Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation 1997, Chromosoma DNA topoisomerases 1996, Annual Review of Biochemistry View all citing articles on ScopusView...
In autopolyploids, there are more than two copies of each chromosome with the same chance to form chiasmata at meiosis. This leads to the formation of multivalent configurations in which chiasma quantification is rather complicated. Here, we present an improved cytological protocol, including ...
Here, we show that this switch protects from chromosome non-disjunction by delaying anaphase entry and promoting TopoIIα-dependent resolution. Full size image Discussion We have defined a mechanism by which the key mitotic kinase, Aurora B, orchestrates safe passage through the metaphase–anaphase ...