Karyotyping is the process of obtaining an image of the complete chromosome complement of a somatic cell. First, cells are obtained and cultured in the lab. A chemical is added to arrest cell division at metaphase when chromosomes are maximally condensed....
Microlamellae join the ends of chromosomes to the fibrous elements of the spindle. The capacity to preserve the intermediate-like filaments is largely dependent upon the use of collidine buffer during fixation. In contrast, during metaphase and anaphase, microtubules are the dominant fibrous element ...
At the start of metaphase, the microtubules arrange the chromosomes in a line along the equator of the cell, known as the metaphase plate (Figure 3b). The centrosomes, on opposite poles of the cell, then prepare to separate the sister chromatids. Anaphase After metaphase is complete, the...
Fig. 1: Deposition of Cnp1 (CENP-A) ceases in metaphase. Fluorescence recovery after photobleaching (FRAP) assays were performed with Cnp1-GFP cells arrested at each stage of the cell cycle. Cells with unbleached centromeres were used as controls. a, c, e Recovery kinetics of Cnp1-GFP fl...
Studies have revealed many roles for Bub1 in both mitosis and meiosis, including the localization of other SAC proteins to the kinetochore, SAC signaling, metaphase congression and the protection of the sister chromatid cohesion. Recent data show striking sex specific differences in the response to...
Interestingly, chromosomes from the older fibroblasts appeared decompacted, indicating that the global chromosome structure may also be impaired during aging (Figure 1C). We also analyzed the CENP-B levels in the metaphase chromosomes and found no significant change with age (Figure 1D). Together,...
Which major phase of mitosis is defined by the split of chromosomal centromeres, followed by the migration of the chromosomes moving to the cellular poles? a. anaphase b. cytokinesis c. metaphase d. telekinesis In which types of cell would mitosis occur?
Watch complete video answer for “Cells show structured chromosomes during (A) Metaphase only” of Biology Class 11th. Get FREE solutions to all questions from chapter CELL CYCLE AND CELL DIVISION.
Watch complete video answer for “In onion root tip during mitotic metaphase, the number of kin” of Biology Class 12th. Get FREE solutions to all questions from chapter CELL CYCLE (CELL DIVISION).
DNA-PKcs À / À cells had a higher proportion of chromosomal misalignment during the metaphase to anaphase transition than did the parental HCT116 cells or the Ligase4 À / À cells (Figure 3d). Abnormal mitotic spindles were further examined via analysis of chromosome lagging in ...