d, e Catenation spread assay analysis. d DLD1 cells induced to express GFP-TopoIIα wild type or mutants were scored for the per cent joined (catenated) chromatids. The mean ± SEM of three experiments where ≥15 cells per experiment were analysed is represented by the graph. (two-...
We showed that Lsd1 KO using an inducible Cas9-SSC line, which was established in our lab recently,21 reduced the proliferation of SSCs significantly (Figure 5d), consistent with its essential role in spermatogenesis.13 Co-immunoprecipi- tation assay showed that an LSD1 polyclonal antibody ...