In Membrane Protein Structure Predictions Methods: Methods and Protocols, expert researcher in the field detail the advances in both experimental and computational approaches of the structure, dynamics and interactions of membrane proteins dividing the volume into two sections. The first section details ...
Membrane protein research has flourished in the past 20 years. Exciting technological innovations in structural biology, including cryoEM single-particle analysis and AI-based protein structure prediction, such as AlphaFold 2, have largely revolutio
Cryo-EM structures of the membrane repair protein dysferlin Dysferlin is essential for plasma membrane repair, and mutations in this protein cause various muscle diseases. Here the authors report the structure of dysferlin, offering insights into its molecular mechanisms and how mutations impair its fun...
A good example of skeletal membrane proteins is spectrin of the human erythrocyte membrane. A distinguishing feature of all integral membrane proteins is that a portion of the mass of the integral membrane protein is integrated into the structure of the lipid bilayer. Integral membrane proteins can...
Improvements in the fields of membrane-protein molecular biology and biochemistry, technical advances in structural data collection and processing, and the availability of numerous sequenced genomes have paved the way for membrane-protein structural geno
Allosteric modulation and G-protein selectivity of the Ca2+-sensing receptor Cryo-electron microscopy structures of the human calcium-sensing receptor in complex with Gi and Gq proteins reveal how this receptor activates distinct G protein subtypes and how its function is modulated by a variety of...
Detergents can also disrupt the structure and function of membrane proteins, as they interfere with inter- and intra-molecular protein–protein interactions.Of ∼8000 known membrane proteins found in human cells, only ∼50 have a determined structure [2]. With membrane proteins being implicated ...
Tim44 is a unique, essential and matrix-localized mitochondrial protein of 44 kDa that has no known homologues in other eukaryotic cellular compartments. As such, it has been the subject of many studies attempting to elucidate its structural and functional properties. Tim44 was discovered in two ...
Biological energy conversion in mitochondria is carried out by the membrane protein complexes of the respiratory chain and the mitochondrial ATP synthase in the inner membrane cristae. Recent advances in electron cryomicroscopy have made possible new ins
surprisingly, they are also the linchpin of the pharmaceutical industry. Although they represent about a third of the known human protein-coding genes, they are targeted by roughly 60% of the currently approved drugs. Unfortunately, at par with their biomedical value is the difficulty of ...