此外,另有研究表明,Gdf11在RTT患者兴奋性神经元和MECP2缺陷小鼠多个脑区中下调,而课题组的研究也显示Gdf11在过表达MECP2(MECP2 TG1)的小鼠模型中显著上调。单细胞核转录组测序显示:Gdf11和Mecp2之间在所有细胞类型中的总体Spearman相关性为0.65,此外,Gdf11和Mecp2在神经元和胶质细胞类型(星形胶质细胞、小胶质...
目的探索甲基-CpG结合蛋白2(methyl CpG binding protein 2, MeCP2)过表达小鼠(MeCP2-Tg1,自闭模型小鼠)发育过程中的听力变化.方法在8周和14周对MeCP2-Tg1小鼠(Tg1组)和WT小鼠(WT组)进行三箱社交行为测试,检测MeCP2-Tg1小鼠的社交功能.在社交行为异常发生前后的5个发育节点(4,8,12,16,20周)记录click及...
Detailed neurobehavioral and electrophysiological studies in transgenic line MeCP2(Tg1), which expresses MeCP2 at approximately 2-fold wild-type levels, demonstrated onset of phenotypes around 10 weeks of age. Surprisingly, these mice displayed enhanced motor and contextual learning ...
The earliest lethality was observed in the highest expression line of MeCP2-TG mice with MeCP2-TG3 mice dying by 3 weeks of age while MeCP2-TG1 mice died between 20 weeks of age and 1 year. Additional experiments were conducted on MeCP2-TG1 mice because of their viability and because...
目的 探索甲基-CpG结合蛋白2(methyl CpG binding protein 2,MeCP2)过表达小鼠(MeCP2-Tg1,自闭模型小鼠)发育过程中的听力变化.方法 在8周和14周对MeCP2-Tg1小鼠(Tg1组)... 闫素梅,钱绍文,胡先丽,... - 《第三军医大学学报》 被引量: 0发表: 2022年 MeCP2蛋白AT-hook 1功能域的破坏导致小鼠行为学异...
auditory brainstem responsehearing abnormalitymethyl-CpG binding protein 2To explore the hearing changes in an autism model mice [MeCP2-Tg1 mice, with overexpression of methyl-CpG binding protein 2 (MeCP2)] during development.Three-chamber social test was used to assess the social ability of Me...
In MECP2-TG1 animals, reducing the levels of Crh or its receptor, Crhr1, suppressed anxiety-likedoi:10.1038/ng.1066Samaco, Rodney CMandel-Brehm, CaleighMcGraw, Christopher MShaw, Chad AMcGill, Bryan EZoghbi, Huda YNature GeneticsSamaco, R.C., Mandel-Brehm, C., McGraw, C.M., et al....
Methods: C57BL/6 mice transgenic for human MeCP2 (B6.Mecp2Tg1) were phenotyped, including autoantibody profiling through antigen array, analysis of cellularity and activation of splenic immune cells through flow cytometry, and measurement of proteinuria. Behavioral tests were condu...
Thus, astrocytic gap junctions facilitate but do not act as a direct trigger for the abnormal neuronal synchrony in young Tg1 mice, revealing the potential role of the astrocyte network in the pathogenesis of MeCP2 duplication syndrome.doi:10.1007/s12264-022-00824-xXia, ShengnanXu, Hua-Tai...
Q-PCR结果进一步表明Gdf11在Mecp2敲除的小鼠中的表达显著降低,而在MECP2 TG1小鼠中增加。这些结果表明Gdf11是MeCP2的敏感基因。 图2|Gdf11与MeCP2表达相关 为了研究MeCP2的缺失如何导致Gdf11的下调的机制,课题组使用核酸酶靶向切割和释放(CUT&RUN)分析野生型、MeCP2敲除和MECP2-TG1小鼠海马中MeCP2的结合...