为了深入研究 MECP2 作为转录因子的功能,首要任务是明确其与 DNA 的结合位点。当前,通过传统的 ChIP-Seq 技术在基因组中精确定位 MECP2 与 DNA 的结合位置存在挑战,这主要是由于技术的分辨率限制,难以精确解析MECP2在DNA中的识别位点...
有意思的是,MeCP2可以作为脚手架蛋白负责这一复合物的组装,数据还提示了这一复合物的形成受到DNA甲基化与新生RNA转录的调控;在MeCP2 KO和T158M小鼠中,MeCP2/Rbfox/LASR复合物发生解离。接着,作者利用RNA-seq、iCLIP-seq、in v...
Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild ...
Our results of miRNA chip assay and ChIP-seq showed that MeCP2 inhibited the expressions of numerous miRNAs by binding to their upstream elements, including not only the promoter but also the distal enhancer. Among the affected miRNAs, miR-22 was identified to remarkably suppress gastric cancer ...
Besides the WGBS and RNA-seq reads generated from promyelocytes and granulocytes, we also analysed WGBS, RNA-seq and MeCP2 ChIP-seq data from the GEO (Gene expression Omnibus) database (Supplementary Table 1). All RNA-seq datasets have been generated from poly-A enriched libraries as publi...
S5B and C. Next, we performed ChIP-Seq assay to identify MeCP2-bound genes in GC cells. A total of 8128 ChIP-Seq peaks with various fold enrichments were obtained, among which 1726 peaks exhibited over 2 folds enrichment. The number of peaks in a region from − 40 kb upstream of ...
ChIP: chromatin immuno-precipitation CREB: cAMP response element-binding protein Crh: corticotropin releasing hormone gene Dnmt: DNA methyl-transferases ELS: early life stress Fos: FBJ murine osteosarcoma FRAP: fluorescence recovery after photobleaching ...
For each group, 17,400 cells were applied to a single lane of the Single Cell A Chip targeting 10,000 cells recovery. Paired-end sequencing (Read1: 26 bp; Read2: 98 bp; i7: 8 bp) of the libraries was conducted using HiSeq2500. The generated data were processed by CellRanger v2.1...
1e). In a genome-wide correlation analysis between MeCP2 and H1, we observed that the MeCP2 ChIP-seq coverage correlated with that of H1 ChIP-seq genome-wide (r = 0.60) (Fig. 1f). In addition, MNase-seq also moderately correlates with MeCP2 ChIP-seq (r = 0.58) and ...