洪炜龙王斯璐白永恒谢洁雅郑建建CN101979658A Sep 9, 2010 Feb 23, 2011 温州医学院附属第一医院 Amplification refractory method for detecting single nucleotide polymorphism of multi drug resistance gene 1(MDR1) by introducing mutation to position-3 of primers...
Recent studies have identified nearly 50% prevalence of the PfMDR1 F1226Y mutation in Thailand and Cambodia, and suggested its contribution to altered antimalarial drug susceptibilities38,39. To examine the role of this mutationin vitro, we employed a single cross-over-based allelic exchange strate...
Quantitative PCR analysis revealed that all parasites continued to express a single copy of pfmdr1, with no change introduced during gene editing (Supplementary Table 2). In vitro drug susceptibility assays were performed on the different pfmdr1 recombinant lines. Assays were performed with two ...
Studies of return- ing travellers as 'sentinels' for drug resistance may be par- ticularly useful, as previously pfmdr1 gene duplication in Africa was first recorded in a returning traveller [56]. For epidemiological purposes, monitoring for mutations in both transporter genes (pfatp6 and pf...
Here, we investigated the role of CD44 in the regulation of drug chemoresistance, using osteosarcoma cells isolated from mice carrying a mutation of the tumor suppressor neurofibromatosis type 2 (Nf2) gene. CD44 expression was knocked-down in the cells using CRISPR/Cas9 approach. Subsequently, CD44...