的人乳腺癌MBA-MB-231细胞观察莪术油对乳腺癌 细胞增殖的抑制作用,并初步探讨其可能的机制,为 莪术油的进一步开发利用提供数据支持。 1 材料与方法 1.1 材料 温莪术药材(浙江永嘉)、人乳腺癌细胞 株MDA-MB-231细胞(ATCC)、胎牛血清(FBS,杭州 四季青生物公司)、RPM1—1640培养基(Sigma公司)、 ...
Percentage of apoptosis improved with combination treatment of myricetin-loaded NLCs and DXT in the MDA-MBA231 breast cancer cells. Expression of antiapoptotic genes (survivin, Cyclin B1, and Mcl1) indicated a significant reduction in factor along with increment in proapoptotic factor Bax and Bid ...
2016年9月,我们探讨EPCR在乳腺癌MBA-MB-231细胞增殖、迁移中的作用及分子机制。现报告如下。 1 材料与方法 1.1 材料 人乳腺癌MDA-MB-231细胞购自中国科学院细胞所,DMEM高糖培养基购自Gibco公司,胎牛血清购自杭州四季青公司,CCK-8试剂盒购自日本株式会社同仁化学,Transwell 小室购自Millipore公司,Lipofectamine2000...
p65miRNA质粒明显下调MDA-MB-231细胞中 p65m R NA的表达水平,并显著抑制细胞中NF-KB的结合活性(P 胞G【 ,/G期细胞比例显著增加,S、 /M期细胞比例明显下降(P<0.05);Westernblot分析结果显示, 沉默p65基因后细胞中cyclinD1蛋白表达下调,p21蛋白表达增加。结论 ...
细胞培养 将人三阴性乳腺癌 MBA鄄MD鄄231 细胞以含 10% DMSO,10% ~ 20% 小牛血清的培养液置于 37 益 ,5% CO2 培养箱培养,次日更换 1 次培养液,倒置显微镜 观察生长情况,取生长良好的对数期细胞用于实验. 转染 72 h 后, 在普通光学显微镜下观察乳腺癌 MDA鄄MB鄄231 细胞,再转到荧光显微镜下观察同一 ...
Mbaveng, E.C.N. Nono, C.C. Simo, M. Zeino, A.E. Nkengfack, T. Efferth Cytotoxicity of seven naturally occurring phenolic compounds towards multi-factorial drug-resistant cancer cells Phytomedicine., 23 (2016), pp. 856-863, 10.1016/j.phymed.2016.04.007 View PDFView articleView in ...
The synthesized SM-AgNPs was evaluated for the anti-cancerous activity on the breast cancer cell line MD MBA 23. The numbers of viable cells were found to decrease with the increasing concentration of the drug. However, the IC50 was achieved for 100 mg/mL with the viability percentage of ...
MDA-MB-453 Cells Suppliers list Company Name:Shanghai Hongshun Biological Technology Co., Ltd. Tel:15901914505 15901914505 Email:3187892335@qq.com Company Name:Shanghai guandao biology engineering co., ltd Tel:18818239863 18818239863 Email:3171921642@qq.com ...
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We found that pharmacologic and genetic inhibition of ACSL1 suppressed TNFα-induced GM-CSF expression in MDA-MBA-231 cells along with the activation of P38 MAPK, ERK1/2, and NF-κB. However, there is no role of β-oxidation and ceramide biosynthesis in TNFα induction of GM-CSF in MDA...