因此,寻找能够抑制Akt/GSK-3β/Cyclin D1/CDK6信号通路的天然产物对抑制TNBC的发生发展具有重要意义。 本研究通过体外实验,观察DP联合siCCAT1对MDA-MB-231细胞增殖、迁移、侵袭及EMT进程的影响,分析CCAT1参与调控的信号通路,探寻DP联合siCCAT...
因此,寻找能够抑制Akt/GSK-3β/Cyclin D1/CDK6信号通路的天然产物对抑制TNBC的发生发展具有重要意义。 本研究通过体外实验,观察DP联合siCCAT1对MDA-MB-231细胞增殖、迁移、侵袭及EMT进程的影响,分析CCAT1参与调控的信号通路,探寻DP联合siCCAT1抑制MDA-MB-231细胞增殖、迁移、侵袭及EMT可能的分子调控机制,从而为以C...
Enhanced Induction of MDA-MB-231 Cell Death using the Combination of Galloflavin and ElectroporationGalloflavinTriple Negative Breast Cancerelectrical pulsesMDA-MB-231 cells.Triple Negative Breast Cancer (TNBC) is difficult to treat since it lacks the three most commonly targeted hormone receptors. ...
复方山慈菇醇提物对人乳腺癌MDA-MB-231细胞增殖与 凋亡的影响* 梁聪1，李媛媛1，莫凡露1，李丹1，周逸帆1，郭哲2△，何萍1△ （1.广西医科大学药学院，南宁 530021；2.广西医科大学第三附属医院急诊科，南宁530031）摘要目的：探讨复方山慈菇醇提物对人三阴性乳腺癌（TNBC ）MDA-MB-231细胞增殖与凋亡的影响。
An autophagy-dependent cell death of MDA-MB-231 cells triggered by a novel Rhein derivative 4FKeywords autophagycell deathRheintriple negative breast cancerTriple negative breast cancer (TNBC) has very poor prognosis and no efficacious therapeutic options due to the absence of a validated molecu...
2.4CSN4表达干扰对于MDA-MB-231细胞凋亡的影响 将CSN4干扰表达实验组和shLuc对照组的乳腺癌MDA-MB-231细胞进行PI染色后用流式细胞仪检测Sub G1期比例,结果显示,CSN4干扰表达实验组的Sub G1期细胞比例相较于shLuc对照组显著增加,CSN4sh3组细胞相比对照组shLuc细胞上调近50%,统计分析表明两者差异极显著(P<0.001)...
人TNBC细胞系MDA-MB-231、高糖DMEM培养基、RIPA裂解液、SDS-PAGE丙烯酰胺凝胶、AV-APC/7-AAD凋亡检测试剂盒,二甲亚砜、OST和洛铂,兔抗人p53、Bax、Bcl2、caspase-3和小鼠抗人β-actin抗体。 Cell viability assay MDA-MB-231细胞根...
本课题组拟构建能用于活体内发光成像检测的人 TNBC 细胞 MDA-MB-231 稳定细胞系,从而为裸鼠脑转移瘤模型的建立提供必晏的基础。 l 材料和方法 1.1材料 1.1.1细胞、质粒及实验动物人乳腺癌 MDA-MB-231 细胞购自 ATCC。人胚肾细胞系 293T、包装质粒 psPAX2 和包膜质粒 pVPack-VSV-G、表达质粒 Lentil F...
To characterize the EVs release, serum-starved TNBC-derived MDA-MB-231 cells were cultured in the presence or absence of 30 µM EGCG for 48 h. A concentration documented to not alter MDA-MB-231 cell viability [45,46,47]. Conditioned media was next collected, and EVs were isolated as ...
glauca against Triple-negative Breast Cancer (TNBC) Cell lines (MDAMB-231). The cytotoxicity of methanolic extract of leaflet with rachis, bark and seeds of S. glauca was examined preliminarily using the MTT assays wherein it was observed that the leaflet with rach...