The human origin triple-negative MDA-MB-231 breast cancer cell line was obtained from the National Centre for Cell Science (NCCS), Pune (MS), India a national facility for providing animal cell lines. The cell line was cultured in DMEM (Cat no-11965092, Thermo Fischer) medium supplemented w...
To investigate the effects of chemotherapy on TNBC cell lines, we first figured out the dose–response curve for cisplatin and paclitaxel of MDA-MB-231 and MDA-MB-468 cells. Next, we identified the appropriate low doses (1.25 μg/ml of cisplatin and 7.5 ng/ml of paclitaxel) and conventio...
The MDA-MB-231 cell line was a kind gift from Dr. Mina Bissell (University of California, Berkeley, CA, USA) and its use was approved by our institute with the establishment of a material transfer agreement. All other cell lines were purchased from the American Type Cult...
To understand the influence of exosomes from TNBC lung metastatic sites on the lung microenvironment, we intravenously injected MDA-MB-231 parental (231 Parental) or BT-549 parental (549 Parental) cells into NCG-HLA-A2.1 mice. Following three in vivo selection rounds, we isolated breast cancer ...
Similar expression patterns of the antagonists, receptors and beta-catenin were observed in the TNBC-derived cell line MDA-MB-231 using the anthracyclines doxorubicin and nogalamycin, suggesting the importance of promoter hypomethylation in chemotolerance. NACT patients showing reduced receptor and/or ...
TNBC cells (MDA-MB-231 and MDA-MB-468 cells) were labeled with CellTracker™ Green. Cell migration was assessed and the results showed that TNBC cells cocultured with CAFs (at a ratio of 2:1) and stimulated with CAF-CM displayed enhanced migration ability (Fig. 1A, B). Cell invasion...
Cell proliferation and apoptosis assay Cell proliferation (MDA-MB-231 and BT549) was tested using the MTT assay. Cells were seeded in the 12 well cell culture plates for 2 weeks, and colony formation rate was calculated. Cell apoptosis was detected using the Annexin V-APC/7-AAD or Annexin...
We used epirubicin to treat MDA-MB-231 (MB-231) cells and found that TGF-beta and breast cancer stem cell markers CD44(+)CD24(-) were increased and were dose-dependent of epirubicin. We established drug-resistant cell line from parental MB-231 cells by chronic treatment with low-...
2A, B). To the contrary, stable FZD5 overexpression remarkably promoted Hs-578t cell growth (Fig. 2D–F). MDA-MB-231 cells with or without FZD5 knockdown were inoculated into nude mice to evaluate the effect of FZD5 on cell growth in vivo. Consistent with the finding in vitro, tumors...
To understand the expression profiles of circRNAs in TNBC, we profiled the circRNAs in MDA-MB-231 cells (TNBC cell line) and their exosomes and compared them with the profiles in MCF-7 cells (non-TNBC cell line, which PR and ER were positive) and their exosomes17. The expression profiles...