图1 MS2/MCP系统成像技术原理示意图 融合表达荧光蛋白的MCP二聚体与MS2茎环结合实现RNA成像. MCP:MS2衣壳蛋白. Figure 1 Schematic diagram of MS2/MCP system 1.1.2 Larson等[13]基于假单胞菌RNA噬菌体的PP7开发了PP7/MCP系统(图2),其标记RNA的原理与MS2/PCP系统相同。噬菌体PP7的外壳蛋白与其同源RNA结构...
This study adapts the MS2-MCP system to visualize endogenous mRNAs in Caenorhabditis elegans. Inactivating nonsense-mediated decay and expressing low levels of cytoplasmic MCP allows the live imaging of functional, cytoplasmic transcripts in the nematode.Tocchini, Cristina...
MS2 coat proteins bound to yeast mRNAs block 5' to 3' degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2‑MCP system[J]. RNA, 2015, 21(8): 1393‑1395. [14]Heinrich S, Sidler CL, Azzalin CM, et al. Stem‑loop RNA labeling can affect ...
3.2 Function Ground (GND) The GND pin is the system ground pin. Ground 3.5 ALERT Output The MCP9800/1/2/3’s ALERT pin is an open-drain output pin. The device outputs an alert signal when the ambient temperature goes beyond the user-programmed temperature limit. 3.6 Address Pins (A2, ...
MS2 systemMS2V6MS2V7Quantitative fluorescence microscopyShort-lived mRNAsSingle cellSingle-molecule imagingsmFISHVisualization of single mRNAs in their native cellular environment provides key information to study gene expression regulation. This fundamental biological question triggered the development of the.....
MCP is the coat protein of MS2 phage, and Csy4 is a small protein involved in crRNA generation of CRISPR 1-F system, which can recognize and bind RNA with high specificity. CRISPR/Cas and other genome editing technologies have some problems, such as large molecular weight of targeted ...
Ansermet, 1211 Geneva-4, Switzerland;elsevierMech DevArib G, Cleard F, Maeda RK, Karch F. 2015. Following the intra- cellular localization of the iab-8ncRNA of the bithorax com- plex using the MS2-MCP-GFP system. Mech Dev 138(Pt 2): 133-140....
3.2 Function Ground (GND) The GND pin is the system ground pin. Ground 3.5 ALERT Output The MCP9800/1/2/3’s ALERT pin is an open-drain output pin. The device outputs an alert signal when the ambient temperature goes beyond the user-programmed temperature limit. 3.6 Address Pins (A2, ...
3.2 Function Ground (GND) The GND pin is the system ground pin. Ground 3.5 ALERT Output The MCP9800/1/2/3’s ALERT pin is an open-drain output pin. The device outputs an alert signal when the ambient temperature goes beyond the user-programmed temperature limit. 3.6 Address Pins (A2, ...
Site-directed mutagenesis was performed at the codon 15 of the MS2 bacteriophage coat protein gene,which had been cloned to the virus-like particles expression vector containing non-self RNA fragment. 以本实验室构建的能够装载外源RNA片段的MS2噬菌体“病毒样”颗粒表达载体为基础,利用定点突变技术将衣壳...