Mcm2-7, the eukaryotic replicative helicase that unwinds dsDNA and is positioned at the vanguard of the replication fork, is likely the commonality among these cellular processes. In my thesis work, I find that ATP hydrolysis in one specific active site (Mcm6/2) is required to mediate the ...
Plant Molecular BiologyHerridge, RP, Day, RC, Macknight, RC (2014) The role of the MCM2-7 helicase complex during Arabidopsis seed development. Plant Mol Biol 86: pp. 69-84Herridge, R.P., Day, R.C. and Macknight, R.C. 2014. The role of the MCM2- 7 helicase complex during ...
Here, through analysis of a unique mcm allele targeting a specific ATPase active site (mcm2DENQ), we show that the Mcm2-7 replicative helicase has a novel DRC function as part of the signal transduction cascade. This allele exhibits normal downstream mediator (Mrc1) phosphorylation, implying ...
In particular, we highlight the function of the MCM2-7 helicase subunit Mcm2 as a histone H3-H4 tetramer chaperone. Disruption of this histone chaperone’s functions affects mouse embryonic stem cell differentiation and can lead to embryonic lethality in mice, underscoring the crucial role of ...
A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that ea
In late mitosis and G1, Mcm2-7 are assembled onto replication origins to license them for initiation in the upcoming S phase. After initiation, Mcm2-7 provide helicase activity to unwind DNA at the replication fork. Here we examine the structure of Mcm2-7 on chromatin in Xenopus egg extra...
The Mcm2-7 Complex Has In Vitro Helicase Activity Helicases unwind duplex DNA ahead of the polymerases at the replication fork. However, the identity of the eukaryotic replicative helicase has been controv... ML Bochman,A Schwacha - 《Molecular Cell》 被引量: 460发表: 2008年 Origin single-...
In eukaryotes, although the Mcm2-7 complex is a key component of the replicative DNA helicase, its association with Cdc45 and GINS (the CMG complex) is required for the activation of the DNA helicase. Here, we show that the CMG complex is localized to chromatin in human cells and describe...
The controlled assembly of replication forks is critical for genome stability. The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. At present
Cell-Cycle-regulated interaction between Mcm10 and double hexameric Mcm2-7 is required for helicase splitting and activation during S phase [J]. Cell Rep, 2015, 13(11):2576-2586. [8]Warner DF, Evans JC, Mizrahi V.Nucleotide metabolism and DNA replication [J]. Microbiol Spectr, 2014, 2(...