Mcm2-7 adopts two conformations--a lock-washer-shaped spiral state and a planar, gapped-ring form--in which Mcm2 and Mcm5 flank a breach in the helicase perimeter. GINS and Cdc45 bridge this gap, forming a topologically closed assembly with a large interior channel; nucleotide binding ...
During the G1-S-phase transition, S-phase specific kinases and a series of replication factors activate the MCM2-7 double hexamer to promote the formation of bidirectional replication forks6,10,13,14. Although helicase loading has been investigated in vitro and in vivo15–17, it is still ...
DNA helicaseDNA repairDNA damageSeed developmentArabidopsisThe MINICHROMOSOME MAINTENANCE 2-7 (MCM2-7) complex, a ring-shaped heterohexamer, unwinds the DNA double helix ahead of the other replication machinery. Although there is evidence that individual components might have other roles, the ...
MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the ...
Mcm2-7 is a key regulatory component of cell cycle progression. (a) Homology model of the human Mcm2-7 complex. No high resolution structure yet exists for the eukaryotic Mcm2-7 complex. However, the archaea have homohexameric Mcm helicases, and a crystal structure of the S. solfataricus...
A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that ea
MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the ...
of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7...
into how this helicase-loading machine is specialized to ensure that pre-RC formation is temporally and spatially restricted in the cell. Results ORC-Dependent ATP Binding and Hydrolysis by Cdc6 Because Cdc6 functions in cooperation with other repli- ...
Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during rep...