Biochemistry The Mcm2-7 Replicative Helicase is Essential to Coordinate DNA replicationCheckpoint Regulation and Sister Chromatid Cohesion UNIVERSITY OF PITTSBURGH Anthony Schwacha TsaiFeng-LingDNA replication is a complex and highly regulated cellular process that ensures faithful duplication of the entire ...
DNA helicaseDNA repairDNA damageSeed developmentArabidopsisThe MINICHROMOSOME MAINTENANCE 2-7 (MCM2-7) complex, a ring-shaped heterohexamer, unwinds the DNA double helix ahead of the other replication machinery. Although there is evidence that individual components might have other roles, the ...
which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability. The essential mini-chromosome maintenance proteins 2–7 (MCM2–7) ...
Here, through analysis of a unique mcm allele targeting a specific ATPase active site (mcm2DENQ), we show that the Mcm2-7 replicative helicase has a novel DRC function as part of the signal transduction cascade. This allele exhibits normal downstream mediator (Mrc1) phosphorylation, implying ...
Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during rep...
The MCM2–7 complex is essential for both the initiation and elongation phases of eukaryotic chromosome replication. There is some evidence that MCM2–7 proteins may act as a DNA helicase; at the same time, a variety of other DNA helicases have also been implicated in the replication of euk...
The Mcm2-7p heterohexamer is the presumed replicative helicase in eukaryotic cells. Each of the six subunits is required for replication. We have purified the six Saccharomyces cerevisiae MCM proteins as recombinant proteins inEscherichia coli and have reconstituted the Mcm2-7p complex from individual...
A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.More Immunogen Recombinant protein Key Feature Clonality Monoclonal Isotype IgG1 Host Species Mouse Tested Applications WB...
In eukaryotes, although the Mcm2-7 complex is a key component of the replicative DNA helicase, its association with Cdc45 and GINS (the CMG complex) is required for the activation of the DNA helicase. Here, we show that the CMG complex is localized to chromatin in human cells and describe...
A high-resolution cryo-EM structure of the heptameric Cdt1–Mcm2–7 complex of the replicative helicase from budding yeast suggests a ‘spring-action’ DNA-unwinding mechanism. The minichromosome maintenance complex (MCM) hexameric complex (Mcm2–7) form