MCF-7 cells are useful for in vitro breast cancer studies as a result of the cell line retaining several ideal characteristics particular to the mammary epithelium. These include the ability for MCF-7 cells to process estrogen in the form of estradiol via estrogen receptors in the cell cytoplasm...
Thus, MCF7 as the luminal A model, MB453 as the HER2 model and MB231 as the triple-negative model were used. No effect on cell growth and viability was detected in MB453 and MB231 cells. In MCF7 cells, hypocholesterolemia (1) reduced cell growth, and Ki67 expression; (2) increased...
Similarly, it was discovered that well-differentiated breast carcinomas have higher levels of ER expression than poorly differentiated breast carcinomas [115]. Conversely, ER-low breast tumours were linked to increased Ki-67 expression, negative PR expression, positive HER2 expression, larger size, an...
Interestingly, separate clusters of BC samples were identified to be varying in CRYAB expression, where CRYAB tended to be less expressed in ER-positive BC but more expressed in ER-negative BC111, suggesting a subtype-specific gene expression pattern in CRYAB. While the tumour-suppressive roles ...
A: MCF_7 cells negative for the expression; B: MCF_7 cells transfected with pcDNA 3.1_HER2 positive for HER2 expression, with the membrane stained brown 图4 HER2 在细胞膜上表达的流式细 胞仪检测 i .4 low ytom tri analysis or sur a xpr ssion o $%&2 ...
HER2+, and triple negative breast cancer (TNBC) categories was not available, IHC data describing ERα, HER2, and PRβ status were downloaded. The tumor samples were separated into the correct intrinsic subtype category based on their positive or negative status of ERα, PRβ, and HER2 as ...
Furthermore, these genes do not show expression differences in Triple-negative Basal cells (MDA468) when compared to MCF10A cells and HER2+ cells (SKBR3) present residual (HOXB8) or null expression (HOXB9). Download: Download high-res image (455KB) Download: Download full-size image Fig....
HER2、resultedinanup.regulationofBCRlPviathe phosphatidylinositoI 3一kinase I3K)/Aktandnuclear factor-kappaB(NF-KB)signaling.TreatmentofMCF/ HER2cellswiththePI3KinhibitorLY294002.theIKB ph0sph0rylati0ninhibitorBayl1.7082,andthedominant negativemutantofIKB仪inhibitedHER2.inducedBCRP promoteractivity.Further...
All cell lines were routinely tested for mycoplasma infection and found to be negative. MCF10A cells with stable overexpression of human ATP13A4 WT or the catalytically dead mutant D486N were produced via lentiviral vector transduction. After lentiviral transduction, cells were selected with 1 μ...
All cell lines were routinely tested for mycoplasma infection and found to be negative. MCF10A cells with stable overexpression of human ATP13A4 WT or the catalytically dead mutant D486N were produced via lentiviral vector transduction. After lentiviral transduction, cells were selected with 1 μ...