适当的培养技术和程序是确保转染成功的重要组成部分。传代培养(也称为传代)是指移除培养基并将培养物中的细胞转移到新鲜的生长培养基中,从而实现细胞增殖。请参阅我们为本实验方案提供的产品列表 传代 将细胞置于 T-75 培养瓶中维持培养。 使用Gibco TrypLE 解离试剂。
Add appropriate aliquots of cell suspension to new culture vessels. Subcultivation Ratio:A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal:Every 2 to 3 daysPreservation:Freeze medium:Complete growth medium supplemented with 7.5% (v) DMSO Storage temperature:liquid nitrogen vapor ...
Conclusions Our studies resulted in the development of a growth protocol where the effects of chronic, physiologically relevant alterations in the microenvironment on cellular transformation were examined. From our results, we were able to propose a model of transformation within the MCF-10A cell line...
The cells were then stained following standard protocol using monoclonal anti- SCIENTIFIC REPORTS | 4 : 4568 | DOI: 10.1038/srep04568 8 www.nature.com/scientificreports RECK, anti-E-cadherin (24E10, Cell Signaling) and anti-N-cadherin antibody as the primary antibody and CF488A goat anti-...
with amino acids in cell culture (SILAC) and have been reviewed elsewhere [11,12]. Among them iTRAQ is a powerful tool in which up to eight samples can be relatively quantified in one experiment thereby reducing inconsistency between analyses [13]. ...
Specifically, 5α-reduced metabolites (5α-pregnanes, shown to stimulate cell proliferation and detachment) are produced at a significantly higher rate in tumorous tissue, indicating increased 5α-reductase (5αR) activity. Conversely, the activities of 3α-hydroxysteroid oxidoreductase (3α-HSO) ...
Several technologies that emerged at the turn of the millennium are available for shot-gun protein expression profiling They include isotope-coded affinity tag(ICAT), isobaric tags for relative and absolute quantification(iTRAQ) and stable isotope labeling with amino acids in cell culture (SILAC)and ...
Cell viability was determined by CellTitre 96 Aqueous One solution assay (Promega) according to the manufacturer's protocol. For acini morphogenesis assays, cells were pre-treated with compounds in 2D 24 h before seeding in compound-free assay media in 3D. Acini were fed with compound-containing...
适当的培养技术和程序是确保转染成功的重要组成部分。传代培养(也称为传代)是指移除培养基并将培养物中的细胞转移到新鲜的生长培养基中,从而实现细胞增殖。请参阅我们为本实验方案提供的产品列表 传代 将细胞置于 T-75 培养瓶中维持培养。 使用Gibco TrypLE 解离试剂。 每3-4 天进行一次细胞传...
The low-intensity ultrasound effects on MCF7 (human breast adenocarcinoma) and MCF10A (healthy breast cells) have been investigated at different sonication protocol to probe the effectiveness and the selectivity of the ultrasound (US) treatment and to understand the implications between cell mortality,...