Dembinski TC, Green CD (1982) Regulation of oestrogen responsive- ness of MCF-7 human breast cancer cell growth by serum concentration in the culture medium. In: Fisher G, Wieser RJ (eds) Hormonally defined media--a tool in cell biology. Springer- Verlag, Berlin, pp 439-442...
The 2 hrs preirradiation incubation with PEI/DNA complex for MCF-7 cells exhibited a significantly enhanced luciferase activity (P<0.01). In addition, the transfection efficiency was also affected by serum, type of solvent and the volume of culture media.Conclusions: These results suggest that ...
Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology ...
To obtain multicellular spheroids from MCF-7 human breast cancer cells we adhered to the following procedure: (a) limiting the adherence of cell to the substratum; (b) seeding more than the minimum number of cells; (c) guaranteeing the presence of estrogens in the culture medium. Charcoal-dex...
Dr. Aliaa Al-Amoudi provided the human breast cancer cells (MCF-7) cells from King Fahad Medical Research Center/ King Abdulaziz University as described previously [50,51,52]. The MCF-7 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) media (Multicell Invitrogen) supplem...
To study the communication of mature adipocytes and breast cancer cells in vivo, we chose 10 metastatic pathologic samples and 10 non-metastatic pathologic samples to do immunostaining.The co-culture media of human MCF-7 breast cancer cells and human mature adipocytes increased motility of MCF-7 ...
Cell culture media (n=5) 82-105% 89-112% 81-106% 82-110% 【特异性】 本试剂盒识别天然和重组牛恶性卡他热(MCF),经检测与其它相似物质无明显交叉反应。由于受到技术及样本来源的限制,不可能完成所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
MCF-7 Culturing Protocol: 1.Use Eagle's MEM, supplemented with 10% FBS, 1% penicillin/streptomycin. Can also add non essential amino acids (0.1 mM), Insulin (10ug/mL) and Sodium pyruvate (1mM). Add 10nM estrogen to media for a 3-4x increase in cell numbers. Maintain temperature at 37...
Cell culture media and sera were obtained from Invitrogen Life Technologies (San Diego, CA, USA). Rabbit polyclonal IgG antibodies against c-IAP1 and XIAP were obtained from Upstate (Merck, Darmstadt, Germany). Protease inhibitor cock- tail tablets were obtained from Boehringer Mannheim (East ...
1990. PubMed: 2334903Pratt SE , Pollak MN . Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993. PubMed: 76...