五、SUBCULTURINGPROTOCOL 1.Removeanddiscardculturemedium. 2.Brieflyrinsethecelllayerwith0.25%(w/v)Trypsin-0.53mMEDTAsolutiontoremovealltracesof serumthatcontainstrypsininhibitor. 3.Add2.0to3.0mlofTrypsin-EDTAsol
Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope un...
Cell Lines B16f10 Cells American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/cell lines b16f10 cells american type...
The induction of immunogenic cell death (ICD) is one of the possible mechanisms behind this event, but the protocol conditions necessary for a robust induction of ICD by PDT have not been defined. In this work, the immunogenicity of B16F10 melanoma cells treated with different PDT protocols ...
4.2. Cell Culture B16F10 melanoma, LLC lung cancer, and CT26 colon cancer cells were purchased from the American Type Culture Collection (Rockville, MD, USA). B16F10 cells were maintained in Minimum Essential Medium (MEM, Welgene, Daegu, Korea) with 100 mL/L fetal bovine serum (FBS) and...
RNA extraction and quantitative real-time PCR (qRT-PCR) RNA templates from cell culture were isolated using RNeasy Micro Kit (QIAGEN) following the manufacturer's protocol. cDNA synthesis was performed with GeneAce cDNA Synthesis Kit (Nippon Gene, Tokyo, Japan). qRT-PCR was performed using ...
Materials and Methods Cell culture Mouse melanoma cell line B16F10 cells were obtained from ATCC and cultured in DMEM (WelGENE Inc.) supplemented PLoS ONE | www.plosone.org 6 February 2012 | Volume 7 | Issue 2 | e31477 Sox10 and Target Genes Control Melanoma Migration...
Depletions of the corresponding cell populations using this protocol were confirmed by a flowcytometric analysis of spleen cells obtained one day after the mAb inoculation (data not shown). Two independent experiments with 5 mice per group were performed. Gene expression analysis Multi-gene expression...
The experiments were carried out in accordance with the protocol of the Ethics Commission of Butantan Institute (CEUAIB N° 638/09). Cell cycle of lung metastasis cells The metastases of lung were removed after necropsies proceed and perfused immediately with 1 ml of ice-cold enzyme solution ...
Gelatin zymography was performed to evaluate the activities of matrix metalloproteinases (MMP-2 and MMP-9) in FN-induced cells both in the presence and absence of cirsiliol according to a protocol described previously [54]. 4.11. Extraction and Estimation of Cellular Proteins Cell extraction ...