protein expressionrecombinant proteinstable cell linestransient expressionRecombinant proteins, such as monoclonal antibodies, are produced in mammalian cell lines to introduce proper protein folding and post-translational modifications, which are essential for full biological activity. In both the industrial ...
SDS-PAGE analysis to evaluate protein expression Transiently transfected expression Transiently transfect cells 2 weeks ① Feasibility report ② If expression purification is feasible, provide a certain amount of protein samples to customers based on the expression situation Cell supernatant affinity purificati...
Protein expression media Stem cell media Neurobiology media Custom cell culture media Are you a Cell Culture Hero? Gibco Cell Culture Heroes spotlights PhD and postdoc researchers across the globe with the primary focus of telling the story of their research. This program promotes education and crea...
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Control of mammalian germ cell entry into meiosis. Mol. Cell. Endocrinol. 382, 488–497 (2014). Article CAS PubMed Google Scholar Soumillon, M. et al. Cellular source and mechanisms of high transcriptome complexity in the mammalian testis. Cell Rep. 3, 2179–2190 (2013). Article CAS ...
SLURP-1 (secreted mammalian Ly-6/urokinase plasminogen activator receptor-related protein-1) is a novel auto/paracrine cholinergic peptide that can bind to... AI Chernyavsky,J Arredondo,V Galitovskiy,... - 《Am J Physiol Cell Physiol》 被引量: 80发表: 2010年 加载更多0...
(HER2) is a type of protein present outside the breast cells, but in the case of BC positive toHER2, this protein promotes the division of cancer cell in breast other than the normal cells(Slamon et al., 2001). Trastuzumab (Herceptin) is a monoclonal antibody, used to treat HER2-...
Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on vario
i Schematic illustration of the construction of cell-type classifiers based on the PROMITAR platform. The mKate fluorescence protein was co-transfected as an internal control. j The MITA and MITR designs showed cooperating effects regarding cell-type classification. HEK293 and Huh7 cell lines were...
Transcriptional programming in multiple mammalian cell types To control gene expression at distinct levels, we built a library of synthetic operators containing 2×–16× gRNA BS for gRNA4, 7, and 10 (Fig. 1c). Transient expression of mKate was assessed in CHO cells at 48 hours post-tran...