protein expression. The vector is ideal for large-scale protein production, especially when used in combination with our FreeStyle™ MAX CHO or 293 Expression Systems. We expressed several proteins and observed 8–30 mg/L of recombinant protein produced using...
The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable ...
Therefore, in this work, we built up a new mammalian expression vector that enhances the feasibility to use mammalian cells to express proteins as biomarkers. Among the modifications, an Igκ signal peptide and a 6xHis tag were inserted into this vector in order to drive the protein to the ...
产品名称:Mammalian Protein Expression - Cloning Gene into Adenoviral Expression Vector 产品型号:C110 产品展商:ABM 产品文档:无相关文档 简单介绍 Mammalian Protein Expression - Cloning Gene into Adenoviral Expression Vector Mammalian Protein Expression - Cloning Gene into Adenoviral Expression Vector的详细介绍...
产品名称: Mammalian Protein Expression - Cloning Gene into Adenoviral Expression Vector 产品型号: C110产品展商: 其他品牌产品文档: 无相关文档 简单介绍 Mammalian Protein Expression - Cloning Gene into Adenoviral Expression VectorMammalian Protein Expression - Cloning Gene into Adenoviral Expression Vector 的...
Expression Vectors can be transfected into mammalian cells and the fusion protein can be easily characterized using commercially available antibodies. The presence of three copies of the epitope in each vector enhances detection of the fusion protein in downstream applications. An expression vector that ...
D Cazalla,JR Sanford,JF Cáceres - 《Protein Expr Purif》 被引量: 76发表: 2005年 Expression and One-Step Purification of Recombinant Proteins Using an Alternative Episomal Vector for the Expression of N-Tagged Heterologous Proteins in P... Here we report the construction of an alternative episo...
Novel vector design and cell engineering approaches will serve to further enhance the value of baculovirus technology. Over the past 20 years the baculovirus–insect cell expression system has become one of the most widely used systems for routine production of recombinant proteins1–6. A number of...
pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res. 18, 5322 (1990). Article CAS PubMed PubMed Central Google Scholar Konno, D. et al. Neuroepithelial progenitors undergo LGN-dependent planar divisions to maintain self-renewability during mammalian neurogenesis. Nat. Cell Biol. ...
Cloning vector (1ug) Expression & purification results QC data 2-3 weeks Transient Transfection Expression Pilot Expression: Expression test Purification test Western blot analysis Scale Up: Protein/Antibody expression (0.2-1L) Purification: affinity chromatography, gel filtration, ion-exchange QC: SDS...