In the metabolic engineering of biochemical pathways, generating unnatural protein fusions between sequential biosynthetic enzymes is a useful method to increase system efficiency and product yield. Here, we reported a special case. The malonyl-CoA reductase (MCR) of Chloroflexus aurantiacus catalyzes the...
12, 13 Given that 4 and 5 are fluorinated at positions associated with the substitution of malonic building blocks, producing these compounds utilizing a biosynthetic approach may represent a sustainable route to produce designer drugs. While malonyl-CoA ligases like MatB represent exciting ...
2. Malonyl-CoA – a vital metabolite 3. Mechanism of malonyl-CoA sensing 4. The architecture of a malonyl-CoA biosensor 5. The design criteria of an effective malonyl-CoA biosensor 6. Applications of malonyl-CoA biosensors 7. Conclusion and future perspectives Acknowledgement ReferencesShow full ...
Type II fatty acid synthesis (FAS II) is an essential process for bacteria survival, and malonyl-CoA:acyl carrier protein transacylase (MCAT) is a key enzyme in FAS II pathway, which is responsible for transferring the malonyl group from malonyl-CoA to the holo-ACP by forming malonyl-ACP. ...
ambofaciens genome contains several genes encoding homologues of CCRCs, albeit not clustered with the stambomycin biosynthetic genes and predicted on the basis of sequence alignments to be incapable of directing the production of pentyl- or hexylmalonyl-CoAs (Supplementary Fig. 1). Thus, the ...
acyl-CoA ligasealkylmalonyl-CoAbiosynthesisenzyme catalysisnatural productpolyketidereveromycinsecondary metabolismUnderstanding the biosynthetic mechanism of the atypical polyketide extender unit is important for the development of bioactive natural products. Reveromycin (RM) derivatives produced by Streptomyces sp...
cerevisiae via the malonyl-CoA pathway. Integration of multiple copies of biosynthetic genes, overexpression of precursor supply genes, and engineering the redox cofactor all improved the 3HP production, resulting in a strain able to produce 3HP at a titer of 9.8 ± 0.4 g L−1 with a yield...
ambofaciens genome contains several genes encoding homologues of CCRCs, albeit not clustered with the stambomycin biosynthetic genes and predicted on the basis of sequence alignments to be incapable of directing the production of pentyl- or hexylmalonyl-CoAs (Supplementary Fig. 1). Thus, the ...
In vitro experiments have demonstrated that polyketide biosynthesis in reconstituted PKS systems requires the fatty acid synthase (FAS) enzyme malonyl CoA:ACP acyltransferase (FabD) from streptomycetes. It has also been shown that holo-ACPs from a type II PKS can catalyze self-malonylation in the ...
The decreased de novo fatty acid synthesis transcription and the increased β-oxidation transcription resulted in the increase of acetyl-CoA and malonyl-CoA. It is the up-regulated spinosad pathway coupling with the enhanced concentration of acetyl-CoA and malonyl-CoA that contributed to the increase...