adding a tag. The expression plasmid for human FLAG-tagged decorin was purchased from GenScript (OHu16408D). Subcloning efficiency DH5α competent cells (Thermo Fisher) were transformed by heat shock and used for plasmid amplification. Qiagen Plasmid Plus Miniprep, Maxiprep or Gigaprep kits were...
epithelial cells on the oral surface of the arms are not elongated (Figure3K,L). Cell shape measurements show a clear reduction in the length to width ratio with a decrease in length of otherwise elongated epithelial cells (n = 40; ANOVA,P < 0.05; ...
The reaction was chilled on ice before adding 5 µl of the mix to chemically competent cells, kindly donated by Dr. Ernesto Ciabatti (MRC Laboratory of Molecular Biology, Cambridge, UK). This was left on ice for 2 min and then plated onto LB plates containing ampicillin. Colonies ...
Synthetic biology also involves the construction of entire genomes for making RNA and proteins, which can then be assembled to form biomolecular complexes, biological pathway systems, organisms, and cells. The current methods of DNA synthesis are too expensive and too slow for assembling long nucleic...
The DpnI-digested PCR products were then directly used for the transformation of chemically competent E. coli 10 beta cells. The preparation of the competent cells and the transformation were performed as described by Green and Rogers [24]. The transformants were selected for kanamycin resistance ...
The objective is to guarantee that the waste management technology is efficient, dependable, and competent to handle the particular difficulties posed by bio-medical waste. 3.1.5. Cost-Effectiveness (CE) The cost-effectiveness criterion focuses on examining the financial elements of managing bio-...
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Using immunotherapy, gene editing can modify a patient’s immune cells, specifically T-cells, to recognize and attack cancer cells; the cancer cells can be made more susceptible to chemotherapy or other treatments, enhancing the efficacy of existing treatments. Personalized cancer vaccines can be ...