which surrounds the proximal base of the arm (Figure2E, arrowheads). Within the epithelium, the basal cells of the Kölliker’s organs have multiplied and formed into cup-shaped invaginations secreting spine-like setae (Figure2E′). The...
The reaction was chilled on ice before adding 5 µl of the mix to chemically competent cells, kindly donated by Dr. Ernesto Ciabatti (MRC Laboratory of Molecular Biology, Cambridge, UK). This was left on ice for 2 min and then plated onto LB plates containing ampicillin. Colonies ...
The DpnI-digested PCR products were then directly used for the transformation of chemically competent E. coli 10 beta cells. The preparation of the competent cells and the transformation were performed as described by Green and Rogers [24]. The transformants were selected for kanamycin resistance ...
Further, autoclaving can be used to treat some waste streams that are chemically contaminated or dangerous, but not all waste streams. The success of autoclaving depends on waste separation and appropriate packing. 3.2.3. Microwaving Managing bio-medical waste alternatively through “Microwaving” is...
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