Methods for lyophilizing competent cells.I method for producing prokaryotic cells which are competent for transformation, said method comprising: (a) growing said cells in a suitable medium for growth; (B) said cells competent; and (c) lyophilizing said competent cells.JESSEE, JOEL, A....
38. A method of preparing the composition of claim 1 comprising: a) mobilizing CD34+ hematopoietic cells into the blood of the subject; b) isolating leukocytes from the subject's blood by apheresis; c) isolating the CD34+ hematopoietic cells from the isolated leukocytes by an immunoselective me...
Opdenakker, C., et al., “Cells Regulate the Activities of Cytokines by Glycosylation”, The FASEB Journal 9:453-457, 1995. Pueyo, J.J., et al., “Degradation of Transport-Competent Destabilized Phaseolin with a Signal for Retention in the Endoplasmic Reticulum Occurs in the Vacuole”,...
Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, the homogeneity of ice thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and ha...
Different regions of RNA molecules can often engage in specific interactions with distinct RNA-binding proteins (RBPs), giving rise to diverse modalities of RNA regulation and function. However, there are currently no methods for unbiased identification
The penetration of substances from the surface to deep inside plant tissues is called infiltration. Although various plant tissues may be effectively saturated with externally applied fluid, most described infiltration strategies have been developed for
must run the notebook, and the outputs of the cells must be printed. Having all cells executed sequentially is a way of showing to us that your notebook correctly produces the displayed output. The absence of this output will be penalised). You can use Google Colab or develop your Jupyter...
Clinicians proposed a sequence of three training modules: (1) prior knowledge (basic operations for iPhone, software, and braille display), (2) methods for preparing a face-to-face discussion, and (3) processes during a face-to-face discussion. Results demonstrate the feasibility of using a ...
CBoth wound healing and cell barrier assay methods are based on preparing a defined gap in a cell layer and monitoring the movement of cells to occupy the empty area over time.DThe Dunn chamber method combines the chemotactic movement of cells and its monitoring over time.EThe Boyden chamber...
An example of the means for preparing the invention polypeptide(s) is to express nucleic acids encoding the invention polypeptide in a suitable host cell, such as a bacterial cell , a yeast cell, an amphibian cell (i.e., oocyte), or a mammalian cell, using methods well known in the art...