1A). Next, we plotted the expression of the general macrophage marker CD68 and the tumor-associated macrophage marker CD163 versus F2R in tumor-only datasets (Fig. 1B). In these sets, CD68 and CD163 expression significantly correlated (Sup. Figure 1), and both markers correlated with PAR1...
4j). Moreover, the mRNA expression of anti-inflammatory marker IL-10 was significantly decreased upon LPS stimulation (Supplementary Fig. 4f). Additionally, PA-treated BMDMs from Noc4lLKO mice also expressed much higher levels of IL-6 and an upward trend of TNFα compared to the age-matched...
Finally, DSP analysis using CD68 morphology marker, revealed that lining and sublining layer CD68pos spatial transcriptomics distinguished, at baseline, remission RA who flared after treatment modification from those who did not.Conclusion Disease flare is a common event in RA in sustained remission ...
(C-G) Quantitative polymerase chain reaction (qPCR) analysis of mean relative mRNA expression of M2 macrophage marker genes in RAW264.7 macrophages transfected with control siRNA or GMFG siRNA for 48 hours, then stimulated without (M0) or with M2 (IL-4/IL-13) macrophage inducers for 24 ...
After staining, the cells were washed and resuspended in FACS buffer (PBS containing 2% FBS) for surface marker staining as described above. FACS analyses were performed using an Attune NxT Flow Cytometer (Thermo Fisher Scientific). Data were analyzed using FlowJo. For measuring uptake of fatty ...
mansoni infected mice contained 'M2'-like RELMalpha +ve cells and expression of the 'M2' marker, Ym1, was also significantly increased (p 0.01), consistent with polarisation induced by infection. In conclusion, we describe a sparse resident murine cardiac macrophage population that is not ...
Local macrophage subpopulations were histologically immuno-phenotyped using CD68 (a ubiquitous macrophage marker) and CD163, a specific M2 macrophage marker. Without occlusion few M2-macrophages reside in the perivascular space. Early after occlusion (12h) the number of M2 macrophages increases strongly...
Six hours after administration, echocardiography was performed to assess the success of the model, with LVFS < 50% as a marker of successful SIMD mouse modeling [13, 39]. Grouping: The mice were grouped randomly as follows (N = 12): (1) control group: injected intraperitoneally ...
FACS analysis of WT and TβRII-/- BMDMs after 11 days in culture with M-CSF, a macrophage growth factor, showed that cells of both genotypes were uniformly Ly6GlowCD11bhi and expressed F4/80, a pan-macrophage surface marker (Additional file 1: Figure S2A), indicating that the purity...
To evaluate the level of miR-21-5p in plasma EVs, EpCAM, a multifunctional transmembrane protein involved in the regulation of tumor cell adhesion, proliferation, migration, stemness and EMT, was used as a marker to identify and isolate tumor-derived EVs [21]. The use of this marker partia...