4j). Moreover, the mRNA expression of anti-inflammatory marker IL-10 was significantly decreased upon LPS stimulation (Supplementary Fig. 4f). Additionally, PA-treated BMDMs from Noc4lLKO mice also expressed much higher levels of IL-6 and an upward trend of TNFα compared to the age-matched...
After staining, the cells were washed and resuspended in FACS buffer (PBS containing 2% FBS) for surface marker staining as described above. FACS analyses were performed using an Attune NxT Flow Cytometer (Thermo Fisher Scientific). Data were analyzed using FlowJo. For measuring uptake of fatty ...
1A). Next, we plotted the expression of the general macrophage marker CD68 and the tumor-associated macrophage marker CD163 versus F2R in tumor-only datasets (Fig. 1B). In these sets, CD68 and CD163 expression significantly correlated (Sup. Figure 1), and both markers correlated with PAR1...
Moreover, cell surface expression of dectin-1, a marker of alternatively activated macrophages, was robustly induced by PGC-1β (Figure S4D). Taken together, these results suggest that cooperation between the IL-4 signaling pathway and PGC-1β is critical in polarizing macrophages to the more ...
FACS analysis of WT and TβRII-/- BMDMs after 11 days in culture with M-CSF, a macrophage growth factor, showed that cells of both genotypes were uniformly Ly6GlowCD11bhi and expressed F4/80, a pan-macrophage surface marker (Additional file 1: Figure S2A), indicating that the purity...
In addition to their LGL-like morphology, many rhM-CSF-induced CD16+ monocytes showed a pattern of size, granularity, and quantitative cell surface marker expression that closely resembled the pretreatment LGL/natural killer (NK) cell population but that did not resemble the pretreatment monocyte ...
infected with HIV-1ADAor infected and treated with 2ART or 4ART regimens. Cells from each tissue were incubated with labeled antibodies for the identification of CD34 and Lineage-1 cellular markers. Lin-CD34+ progenitor cells were determined by the expression of CD34 marker, excluding all Lineage...
mansoni infected mice contained 'M2'-like RELMalpha +ve cells and expression of the 'M2' marker, Ym1, was also significantly increased (p 0.01), consistent with polarisation induced by infection. In conclusion, we describe a sparse resident murine cardiac macrophage population that is not ...
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In magnetic bead–conjugated antibody cell isolation, antibodies specific to a cell-specific surface marker are attached to magnetic beads, and the cell suspension is incubated with these beads. Once the cells that feature the selected surface marker are bound to the bea...