基于化学检测的分析主要有两种类型,一种是化学还原法和逆转录方法,如m7G MAP-seq、m7G-seq。m7G-seq利用化学还原和脱氨作用选择性将m7G位点转化为碱性位点,并通过逆转录酶成功检测mRNA中的m7G信号。m7G MAP-seq通过硼氢化盐将m7G位点还原为碱性位点,通过逆转录直接记录为cDNA突变并测序。另一种是化学裂解介导的检测...
这里,作者提出m7G突变分析测序(m7G-map-seq),它允许在单核苷分辨率下高通量检测m7G修饰。在该方法中,m7G修饰位置通过硼氢化钠还原转化为碱基位点,通过逆转录和测序直接记录为cDNA突变。作者证明m7Gmap-seq能够有效地检测到已知的m7G在rRNA中的修饰,其突变率高达25%,并且在拟南芥SSU-rRNA中的1581位点绘制了一个先前...
对于人类m7G位点数据,根据其分析检测技术进行了进一步的分类,包括基于单碱基分辨率技术获得的位点(m7G-seq和m7G-MaPseq:8402个位点),以及基于m7G分布检测技术获得的位点(m7G-miCLIPseq:22783个位点;m7G-MeRIP-seq:138534个位点)。对于从直接RNA测序得到的数据,总共收集到了172692个带有m7G注释的鸟苷修饰位点,覆盖了21...
为了阐明Mettl1驱动心肌肥厚的机制,我们采用RNA-seq结合m7G MeRIP-seq,分别在Mettl1过表达的小鼠心肌和NMCM中进行全转录组分析和m7G位点定位。Mettl1过表达后,NMCM中总m7G甲基化水平更高,而Mettl1敲低则具有相反的效果。m7G MeRIP-seq分析显示,在过表达Mettl11的心脏中,有3085个上调基因和5372个高甲基化峰。此外,...
优势三:MeRIP-seq全面升级,spike in严格质控IP实验 云序m6A meRIP-seq Plus(spike in)通过在实验过程中加入两类人工合成的spike in:阳性Spike-In(带m6A修饰)和阴性Spike-In(不带修饰),与样品一起进行MeRIP实验及后续测序分析,根据spike in的检测信号对MeRIP实验进行质控,确保实验流程的准确性、可重复性及抗体的特...
(BoRed-seq) technology. Here, the m7G encourages the creation of G-quadruplexes in miRNAs and aids in the processing of precursor miRNAs [6]. However, it is worth noting that m7G modification was not detected in miRNAs including human Let-7e utilizing m7G-maP-seq by Enroth et al. [18]...
5l). Both the RNA-seq and proteomic data revealed significantly lower levels of IgM in Mettl1-deleted B cells than in control B cells (Supplementary Fig. 12d). To compare the mRNA and protein abundance of BCR signaling pathway-related genes, we performed heatmap analysis using RNA-seq and ...
E, F Radar map (E) and heatmap (F) showed the comparison of RNA modification levels in mRNA in METTL1 knockdown and control THP-1 cells. G Comparison of m7G modification level on mRNA in METTL1 knockdown and control THP-1 cells. Data were presented as mean ± SEM (Student’s t...
A The R2 genomics analysis and visualization platform showed a heatmap of correlations between METTL1 mRNA levels, COG risk, INSS stage, and MYCN status in NBL samples from the GEO database. B The GEO database showed the mRNA level of METTL1 in NBL samples at different stages (except ...
The differentially expressed genes (DEGs) were screened according to the criteria of |Fold change| > 2 and p-value < 0.05, and the DEGs were displayed in a heat map (Fig. 1A, Supplementary Table 5). Based on the results of differential genes, we found that METTL1 expression was ...