高通量测序目前主要以m6A-seq/MeRIP-seq为主,它是目前研究m6A修饰使用最广泛的技术之一。 MeRIP-seq将甲基化DNA 免疫共沉淀(methylated DNA immunoprecipitation, MeDIP)技术、RNA 结合蛋白免疫共沉淀(RNA immunoprecipitation,RIP)技术和RNA 测序(RNA sequencing,RNA-seq)技术组合起来,高精度地检测全基因组(或全转录组...
MeRIP-seq 技术将甲基化DNA 免疫共沉淀(methylated DNA immunoprecipitation, MeDIP) 技术、RNA 结合蛋白免疫共沉淀(RNA immunoprecipitation,RIP)技术和RNA 测序(RNA sequencing,RNA-seq) 技术组合起来,高精度地检测全基因组(或全转录组)范围内的RNA 甲基化。MeRIP-seq 技术采用免疫共沉淀方法,即甲基化RNA 特异性抗体...
[5] Meng J, Cui X,et al., Exome-based analysis for RNA epigenome sequencing data. Bioinformatics. 2013 Jun 15;29(12):1565-7. [6] Meng J, Lu Z,et al.,A protocol for RNA methylation differential analysis with MeRIP-Seq d...
[5] Meng J, Cui X,et al., Exome-based analysis for RNA epigenome sequencing data. Bioinformatics. 2013 Jun 15;29(12):1565-7. [6] Meng J, Lu Z,et al.,A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods. 2014 Oct 1...
[5] Meng J, Cui X,et al., Exome-based analysis for RNA epigenome sequencing data. Bioinformatics. 2013 Jun 15;29(12):1565-7.[6] Meng J, Lu Z,et al.,A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods. 2014 Oct 1...
最早发现的RNA甲基化修饰包括假尿苷和5mC等。在mRNA中,常见的修饰包括m6A、m1A、5mC等。早在上世纪70年代,人们已经在真核生物的mRNA和lncRNA中发现了m6A修饰。但是受到技术手段的限制,检测m6A尤其是对m6A进行定量,甚至是从单碱基水平鉴定m6A,一直进展缓慢。随着高通量测序技术(next generation sequencing, NGS)的发...
12. Simpson, J. T. et al. Detecting DNA cytosine methylation using nanopore sequencing.Nat. Methods14, 407–410 (2017). 13. Garalde, D. R. et al. Highly parallel direct RNA sequencing on an array of nanopores.Nat. Methods1...
m6A敏感性MazF酶切法测序(RNase MazF-sequencing ,MAZTER-seq)和RNA修饰靶标测序(DART-seq)可以同时对转录组范围的m6A甲基化进行单核苷酸分辨率和化学计量定量。在MAZTER-seq中,未甲基化ACA motifs上游用细菌RNase(MazF)选择性酶切,且ACA reads与m6A丰度相关,但这种方法在哺乳动物中只有16%-25%的m6A位点可以被捕获...
m6A methylation in myocardial tissue of septic mice analyzed using MeRIP/m6A-sequencing and RNA-sequencingMAPK11TRAF3IP2Septic cardiomyopathyN6-methyl-adenosineDifferentially expressed geneSeptic cardiomyopathy is a secondary myocardial injury caused by sepsis. N6-methyl-adenosine (m6A) modification is ...
[5] Meng J, Cui X,et al., Exome-based analysis for RNA epigenome sequencing data. Bioinformatics. 2013 Jun 15;29(12):1565-7. [6] Meng J, Lu Z,et al.,A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods. 2014 Oct ...