Plos One上面有篇2015年的文章《Novel Markers to Delineate Murine M1 and M2 Macrophages - PubMed [5]》,比对了野生型C57BL/6J小鼠M0、M1、M2巨噬细胞转录产物的差异(上调、下调),再经过验证,发现在流式上,CD38/Egr2可很好的区分M1和M2,见下图,当然CD38/iNOS也是非常好的区分标记。虽然是7年前,但是也被...
improve the reprogramming of inflammatory macrophages into anti-inflammatory cells to control disease. 巨噬细胞是先天性免疫细胞,它们采用不同的激活状态来响应其微环境。通过促进炎症 (M1) 巨噬细胞向抗炎 (M2) 巨噬细胞的复极化来编辑巨噬细胞激活以抑制炎症性疾病是人们非常感兴趣的。在这里,我们发现小鼠和人...
Phenotypic polarization of macrophages is regulated by a milieu of cues in the local tissue microenvironment. Although much is known about how soluble factors influence macrophage polarization, relatively little is known about how physical cues present in the extracellular environment might modulate proinfla...
Since the early stage of MMs, M1 intratumoral macrophages were fewer than the M2 population; their recruitment was rapidly and progressively overwhelmed by an increase in M2 TAMs during MM progression. Independent of their intratumoral distribu...
在降维聚类分群后的umap图里面,M1和M2它们的界限并不是很清晰,而且在气泡图里面CD86和CD163的表达量也不是说在CD86和CD163里面互斥,仅仅是表达量的些微高低而已。 能泾渭分明的巨噬细胞分类 2022的一篇CELL文章:《Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast canc...
M2巨噬细胞又称替代性活化巨噬细胞(Alternatively activated macrophages, M2)，主要由IL-4炎症因子激活，主要通过分泌IL10等抗炎细胞因子抑制M1巨噬细胞，在对寄生虫、组织修复、血管生成、伤口愈合和过敏性疾病的反应中起着核心作用。M2巨噬细胞可大量释放VEGF、Arg-1、PDGF、TGF-β和IL-10等细胞因子，同时，可...
Types of Macrophages –(M1 and M2) Macrophages are a highly diverse cell population that can assume different phenotypes and functions in response to local environmental cues. Here are some of the main types of macrophages: 1. Classically activated macrophages (M1):These macrophages are induced by...
浸润肿瘤组织或聚集在实体肿瘤微环境中的巨噬细胞被定义为肿瘤相关巨噬细胞(tumor-associated Macrophages, TAMs)。血液中的单核细胞在各种趋化因子和细胞因子的作用下募集到肿瘤细胞周围,从而成为肿瘤相关巨噬细胞。作为肿瘤微环境的重要组成部分,TAMs影响肿瘤生长、肿瘤血管生成、免疫调节、转移和化疗耐药。
M2 macrophages (CD68, CD74, and CD163); 可以看到在umap图里面,M1和M2它们的界限并不是很清晰,而且在气泡图里面CD86和CD163的表达量也不是说在CD86和CD163里面互斥,仅仅是表达量的些微高低变动: umap图和气泡图 如果你对umap图和气泡图不了解,需要去看看单细胞数据分析里面最基础的就是降维聚类分群,参考前...