Cells are infected with RSV, subjected to single and tandem chemical inhibition of key molecular apoptotic, pyroptotic, necroptotic, and autophagic targets, and quantitatively assessed for lytic cell death using a lactate dehydrogenase assay on the supernatant. The results of these studies indicate ...
Programmed cell death is a critical aspect of the innate immune response and controls infections by killing and clearing infected cells, thereby depriving intracellular pathogens of their replicative niche9. Cell death pathways can either induce cell lysis and inflammation, as observed in both pyroptosis...
Plasma membrane rupture (PMR) is the final cataclysmic event in lytic cell death. PMR releases intracellular molecules known as damage-associated molecular patterns (DAMPs) that propagate the inflammatory response1,2,3. The underlying mechanism of PMR, however, is unknown. Here we show that the ...
Programmed-cell death is an antimicrobial defense mechanism that promotes clearance of intracellular pathogens. Toxoplasma counteracts host immune defenses by secreting effector proteins into host cells; however, how the parasite evades lytic cell death
, 2002). Even though RTA has a DNA-binding domain, direct binding of RTA to its promoter is not required for it autoactivation as Oct-1–RTA complex was not detected in gel shift assay and a defective DNA-binding RTA mutant was capable of autoactivating through binding with RBPJκ (...
QIAGEN 1027298 AllStars Cell Death Contr.siRNA (5nmol)QIAGEN 9019341 LED, yellow (585), RG6QIAGEN 9234712 Board, level sensor, BR8000 v1.02QIAGEN 9020469 Peltier module, GSCQIAGEN 9234541 Pumphead click'n'go; HSPS; BR3/9604QIAGEN 9234462 Coupling, CPC, inline, male, 8mmQIAGEN 9234406 Valve...
ABH3 induced cell death in an apoptosis-independent manner through the lytic properties of the peptide that caused disruption of cell membrane. Our results showed that charge tuning and conformation constraining in a lytic peptide could be applied to optimise the anticancer activity of lytic peptides...
In order to estimate the concentration of PGE2 in cell culture supernatant, ELISA (Enzyme-linked immunosorbent assay) was performed using Prostaglandin E2 EIA monoclonal kit (Cayman Chemicals, Ann Arbor, MI, Cat no. 514010) following manufacturer׳s instructions. The ELISA was performed in triplica...
Phage inhibition assay The inhibitory potential of phage ASUmrsa123 on the MRSA host was tested in liquid media. Phage could effectively inhibit the growth of S. aureus MRSA OQ564500 after 100 min when the MOI was 0.01; the bacterial growth started declining and reached complete inhibition after...
Osteolytic lesions and pathological fractures are hallmark manifestations of multiple myeloma (MM), profoundly influencing the quality of life and self-care ability of MM patients. By analyzing transcriptome data and single-cell RNA-seq data from our cen