Methods LysM + immune cells were depleted with diphtheria toxin (DTX) in LysMCre +/- iDTR +/- mice for 6 days prior to noise exposure (mean SPL; 72dBA) and 4 days concurrently. Blood pressure was determined by tail cuff method. Endothelial dysfunction was measured with isometric tension ...
Noise induced a significant increase in expression ofeNOS, VCAM-1, andNOX-2mRNA as well as NOX-2 protein as previously reported [28]. While there was a reduced expression foreNOSandNOX-2in DTX-treated LysMCreiDTRmice, there were no increases in expression in LysM+cell-depleted mice in r...
Mice expressing a Cre recombinase from the lysozyme M-encoding locus (Lyz2) have been widely used to dissect gene function in macrophages and neutrophils. ... KM Vannella,L Barron,LA Borthwick,... - 《Plos Pathogens》 被引量: 49发表: 2014年 Regulation of prostaglandin synthesis during differ...
To determine whether IKKβ was deleted in this preserved Ly6Cpopulation, we repeat the analysis with pups of Lys-M cre IKKβmice bred with mT/mG mice to identify the specific cell population undergoing cre-recombination, as they express GFP. We found that, in dam fed pups, LysM-cre ...
Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages. Blood 96:719-726.Faust, N., Varas, F., Kelly, L.M., Heck, S., and Graf, T. (2000). Insertion of enhanced green fluorescent protein into the lysozyme ...
In the AOM/DSS model, endoscopic monitoring of tumor progression revealed a lowered number of adenomas within the colon of LysMCre/ ;Nod2fl/fl mice, associated with less expression of Tgfb. Mechanistically, lysozyme M was required for the improved disease severity in mi...
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