(6-carboxy-spermyl)-propylamid), Chol-Betaine (Cholesteryl-oxycarbonylmethyl-trimethyl-ammonium), DOSPA (Dioleoyloxy-sperminecarboxamido-ethyldimethyl-propanaminiumtrifluoroacetate), SAINT (Pyridinium amphiphiles analogues) and GL-67 ([Cholest-5-en-3-ol(3b)-3-[(3-aminopropyl)[4-[(3-aminopropyl)...
However, little is known about the interaction between M.tb and macrophage plasmalogens, a subclass of glycerophospholipids with a vinyl ether bond at the sn-1 position of the glycerol backbone. Lysoplasmalogens, produced from plasmalogens by hydrolysis at the sn-2 carbon by phospholipase A2, are...
of LPCAT3 proteins were removed with 3C protease, and the LPCAT3 protein was further purified by size-exclusion chromatography using Superdex 200 Increase 10/300 GL column in a mobile phase containing 150 mM NaCl, 20 mM HEPES, pH 7.5, 1 mM TCEP and 0.01% (w/v) GDN. To purify...
FIG. 16 depicts plates showing LysoPAF Sensitivity test of a yor175c mutant strain transformed with empty pYES2.1, pYES2.1::AtLPCAT1 and pYES2.1::LPCAT2. Cells were grown first in SC-URA+2% glucose media overnight then in protein expression induction media for six hours. Cultures were ...
In these patients, it could be indicated to test LAL activity, using the dried blood spot (DBS) test. The DBS is a simple test used to determine LAL activity by comparing total lipase activity to lipase activity in the presence of a highly specific inhibitor (Lalistat 2) of LAL. It al...