在小鼠BM、血液和脾脏中,FLT3+CD11c细胞在传统的单核细胞片段中鉴定并表达DCs特异性ICAM-3捕获非整联素(DC-SIGN或CD209a),被认为是单核细胞亚群(R2细胞),产生单核细胞来源的dcMildner等人还发现小鼠血液中的Ly6Cint细胞亚群表达DC特...
其一,(A) Ly6GHi CD11b + 细胞(中性粒细胞); 其二,(B) Ly6CLo - neg Ly6G - SSCHi 细胞(嗜酸性粒细胞)且 MHC Class II - 与 CD115 - (详见附加材料 Fig. 5); 其三,(C) Ly6CLo - neg Ly6G - SSCLo 细胞(单核细胞 / 巨噬细胞)且 MHCII + /- 和 CD115 - (以红色柱状图呈现); 其四,(...
UVB-induced melanocyte proliferation in neonatal mice driven by CCR2-independent recruitment of Ly6c(low)MHCII(hi) macrophages. J Invest Dermatol 2013;133:1803-12.Handoko HY, Rodero MP, Boyle GM, Ferguson B, Engwerda C, Hill G, Muller HK, Khosrotehrani K, Walker GJ. UVB- induced ...
7. 小鼠肠道组织 肠道组织里,很多老师都会研究DC细胞,但是DC细胞的标志物CD11C,MHCII在肠道巨噬细胞中也会有表达,老师们在检测和数据分析圈门时要注意哦。 8. 小鼠皮肤组织 除了CD11B,F4/80marker外,可添加EPCAM,CD64,CD24等标...
Using multimarker flow cytometry and RNA expression array analyses on macrophage subpopulations from wound granulation tissue, we identified a Ly6cloMHCIIhi“noninflammatory” subset that increased both in absolute number and proportion during normal wound healing and was missing in Ob/Ob and MYD88-/...
Pacific orange-conjugated anti-IA/IE; PE-conjugated anti-CD4 or Ly6C; PerCP-Cy5.5–conjugated anti- Ly6C or CD8; PE-Cy7–conjugated mAb to anti-CD11c; allophycocyanin-Cy7-conjugated anti- MHCII, B220 and Ly6G; and allophycocyanin-conjugated anti-CD64 and anti-SIINFEKL/H-2Kb (clone 25...
In the tumor, M-MDSC can differentiate into tumor-associated macrophages (TAM) (CD11b+ Ly6Clo F4/80+ MHCII+)17. In tumor-bearing host, myeloid cells are recruited to tumor sites by chemokines produced by the tumor, such as CCL2 and CCL518,19. In chronic tumor-associated inflammation, ...
FACS analysis suggested that decreased Ly6c lo MHCII hi AMs might make the major contribution to attenuated fibrogenesis in RBP‐J cKO mice, probably by reduced inflammatory factor release and enhanced matrix metalloproteinases expression. Using clodronate‐mediated macrophage depletion in RBP‐J ckO ...
In addition to now discernible expression of MHCII and CD11c, monocyte-derived Ly6Clo cells differed from the acute Ly6Chi effector monocytes in that they downmodulated expression of proinflammatory effectors and pathogen sensors. In contrast, these cells gained with increased CCR7 expression the ...
respectively. Monocytes were selected and finally plotted for F4/80 and Ly6C. Herein, Ly6Chighand Ly6Clowmonocytes and macrophages could be selected dependent on the tissue. Monocyte subsets were identified as (B220/CD90/CD49b/NK1.1/Ly6G)lowCD11bhigh(F4/80/MHCII/CD11c)lowand further distinguis...