其一,(A) Ly6GHi CD11b + 细胞(中性粒细胞); 其二,(B) Ly6CLo - neg Ly6G - SSCHi 细胞(嗜酸性粒细胞)且 MHC Class II - 与 CD115 - (详见附加材料 Fig. 5); 其三,(C) Ly6CLo - neg Ly6G - SSCLo 细胞(单核细胞 / 巨噬细胞)且 MHCII + /- 和 CD115 - (以红色柱状图呈现); 其四,(...
Reduced Il17a expression distinguishes a Ly6c(lo)MHCII(hi) macrophage population promoting wound healing. J Invest Dermatol 2013; 133: 783-92.Rodero, M.P.; Hodgson, S.S.; Hollier, B.; Combadiere, C.; Khosrotehrani, K. Reduced il17a expression distinguishes a ly6clomhciihi macrophage ...
Using multimarker flow cytometry and RNA expression array analyses on macrophage subpopulations from wound granulation tissue, we identified a Ly6cloMHCIIhi“noninflammatory” subset that increased both in absolute number and proportion during normal wound healing and was missing in Ob/Ob and MYD88-/...
transgenic DsRED–L. donovaniparasites, we looked for infected cells after 24h of inoculation in the peritoneal cavity. We found three subsets infected withL. donovani. The cells harboring more parasites were Ly6ChiLy6G−CD11b+. The second population of infected cells consisted of Ly6C+ Ly6G+...
These CD45intCD11bint MPCs were further identified as F4/80+MHCII+CX3CR1+Ly6C- cells, comprising ~17% of total CD45+ cells in normal mouse kidney (P < 0.01) and virtually absent from all other organs examined except the heart. Systemic clodronate treatment had more significant depletive...
In light of our transfer results (Figure 3A), these data suggest that the monocyte graft gives rise to Ly6Chi effector monocytes and migratory Ly6Clo cells sequentially that upregulate MHCII expression and hence seem to acquire APC potential. To directly address this point, we next tested the ...
FACS analysis suggested that decreased Ly6c lo MHCII hi AMs might make the major contribution to attenuated fibrogenesis in RBP‐J cKO mice, probably by reduced inflammatory factor release and enhanced matrix metalloproteinases expression. Using clodronate‐mediated macrophage depletion in RBP‐J ckO ...
In the tumor, M-MDSC can differentiate into tumor-associated macrophages (TAM) (CD11b+ Ly6Clo F4/80+ MHCII+)17. In tumor-bearing host, myeloid cells are recruited to tumor sites by chemokines produced by the tumor, such as CCL2 and CCL518,19. In chronic tumor-associated inflammation, ...
Pacific orange-conjugated anti-IA/IE; PE-conjugated anti-CD4 or Ly6C; PerCP-Cy5.5–conjugated anti- Ly6C or CD8; PE-Cy7–conjugated mAb to anti-CD11c; allophycocyanin-Cy7-conjugated anti- MHCII, B220 and Ly6G; and allophycocyanin-conjugated anti-CD64 and anti-SIINFEKL/H-2Kb (clone 25...
In terms of cell surface phenotype, L-DC were initially characterized as a CD11bhiCD11cloMHCII-Ly6C-Ly6G- subset in murine spleen. Their expression of CD43, lack of MHCII, and a low level of CD11c was shown to best ... YY Hey,JKH Tan,HC O'Neill - Wiley-Blackwell 被引量: 9发表...