荧火素酶互补实验(Luciferase Complementation Assay, LCA)作为一种强大而灵活的互作技术,已在多个生命科学研究领域彰显其重要价值,而当我们将目光聚焦到植物科学研究领域,LCA的应用更是展现出了非凡的意义和影响力,尤其是在探索植物如何应对各种逆境胁迫(如干旱、盐碱、极端温度、病原菌侵染等)及其内在的抗性机制方面表...
The Luciferase Complementation Assay (LCA) is an advanced, highly sensitive method designed for studying protein-protein interactions. In this assay, luciferase is split into two fragments: the N-terminal fragment (NLuc) and the C-terminal fragment (CLuc). Each fragment is fused to a different ...
Luciferase complementation assay (LCA) enables detection of protein–protein complementation via bioluminescence following complementation of N- and C-terminal luciferase fragments (NEluc and CEluc) fused to target proteins, but it is necessary to co-express the two genes. Here, we developed LCAs ...
5. Is the firefly luciferase complementation assay quantitative? The mechanism of complementation of the firefly luciferase activity Some of the previously published articles consider the data obtained through the firefly LCA (FLCA hereafter) to be a quantitative measurement of the interaction. Unlike Bi...
In this study we have adapted a split-luciferase complementation assay (LCA) for detecting the assembly of the voltage-gated Na+ (Nav) channel C-tail ... A Shavkunov,N Panova,A Prasai,... - 《Assay Drug Dev Technol》 被引量: 63发表: 2012年 A novel luminescent biosensor for rapid mo...
It consists in a luciferase complementation assay (LCA) enabling to dynamically monitor β゛rrestin2 recruitment at the β2 adrenergic receptor. It allows students to characterize the competitive antagonism of propranolol by calculating pEC50, pKB, and pA2 values....
Here, we developed an in vitro split luciferase complementation-based assay to speed up screening of PA–PB1 interaction inhibitors. By screening 10,000 compounds, we identified two PA–PB1 interaction inhibitors, R160792 and R151785, with potent and broad-spectrum antiviral activity against a ...