Cytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the ...
Cytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the de...
A novel thermostable beetle luciferase based cytotoxicity assay Cytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and.....
The first part is the development of models to differentiate between acute cytotoxicity (AC) and paracellular permeabi... R Konsoula - Dissertations & Theses - Gradworks 被引量: 136发表: 2006年 A comparative study using a fluorescence-based and a direct-count assay to determine cytotoxicity in...
Correlation between luminescence intensity and cytotoxicity in cell-based cytotoxicity assay using luciferase correlation between them, beetle luciferase was stably expressed in human hepatoma HepG2 cells harboring the multi-integrase mouse artificial chromosome vector... S.,Wakuri,K.,... - 《Analytical...
15). Next, we compared the cytotoxicity of the prodrug with LUPIN at various concentrations of furimazine. In the absence of furimazine, LUPIN did not trigger BRET and the prodrug remained caged. At all tested prodrug concentrations, there was a clear dose response of toxicity based on ...
A Real Time Metridia Luciferase Based Non-Invasive Reporter Assay of Mammalian Cell Viability and Cytotoxicity via the ??-actin Promoter and Enhancer Secreted reporter molecules offer a means to evaluate biological processes in real time without the need to sacrifice samples at pre-determined endpoints...
The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. ...
Fig. 4: SARS-CoV-2-Nluc-based antiviral screening. a Three-day cytotoxicity of chloroquine on Vero E6 and A549-hACE2 cells. b Three-day cytotoxicity of remdesivir on Vero E6 and A549-hACE2 cells. c EC50 of chloroquine against SARS-CoV-2-Nluc on Vero E6 and A549-hACE2 cells. d EC...
The present invention also includes measuring the levels of a second enzyme using the modified recombinant luciferase protein by use of alternate luciferin-based analogs. In this manner, the luciferin-luciferase assay has been used for measurement of protease, peptidase, phosphatase, sulfatase, dealkyla...