Cytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the de...
Cytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the ...
www.nature.com/scientificreports OPEN A Fast and Sensitive Luciferase- based Assay for Antibody Engineering and Design of Chimeric Antigen Receptors Venkatesh Natarajan 1,2, Ramakrishnan Gopalakrishnan 1,2, Hittu Matta1,2, Sunju Choi1,2, Songjie Gong1, Alberto ...
While analyzing its cellular effects using a luciferase-based viability assay, we discovered that TU100 disrupts bioluminescence by inhibiting luciferase activity. Unlike other known inhibitors of the firefly enzyme (such as resveratrol), TU100 also inhibited luciferase derived from the sea pansy (R. ...
15). Next, we compared the cytotoxicity of the prodrug with LUPIN at various concentrations of furimazine. In the absence of furimazine, LUPIN did not trigger BRET and the prodrug remained caged. At all tested prodrug concentrations, there was a clear dose response of toxicity based on ...
The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. ...
2.9. EdU incorporation assay PANC-1 cells were planted in 35 mm glass-bottom dishes (Cellvis, D35-20-1-N) and subjected to the indicated treatments. kFluor488 Click-iT EdU Imaging Kit was utilized to measure cell proliferation based on the manufacturer's instructions. Briefly, EdU (20 μ...
Fig. 4: SARS-CoV-2-Nluc-based antiviral screening. a Three-day cytotoxicity of chloroquine on Vero E6 and A549-hACE2 cells. b Three-day cytotoxicity of remdesivir on Vero E6 and A549-hACE2 cells. c EC50 of chloroquine against SARS-CoV-2-Nluc on Vero E6 and A549-hACE2 cells. d EC...
The present invention also includes measuring the levels of a second enzyme using the modified recombinant luciferase protein by use of alternate luciferin-based analogs. In this manner, the luciferin-luciferase assay has been used for measurement of protease, peptidase, phosphatase, sulfatase, dealkyla...
Lee, J-C., “Development of a cell-based assay for monitoring specific hepatitis C virus NS3/4A protease activity in mammalian cells,” Anal. Biochem. (2003) 316(2):162-170. Li, I.T. et al., “Protein biosensors based on the principle of fluorescene resonance energy transfer for mon...