time spent ever, using a different technique Toran-Allerand et al (2005) freezing for 3 min before the first tone presentation was has shown that 17a-estradiol is undetectable in serum, also recorded as an index of whether the groups differed in but is found in the brain of adult mice. ...
After probe insertion, the animal was placed into an open field (21 × 21 × 30 cm). Water was provided ad libitum and room temperature maintained between 19 and 22 ºC. Samples were collected every 20 min. Three samples were taken as baseline and the neurotransmitters dopamine (...
1.A liquid combination vaccine comprising antigens for protecting a subject against at least diphtheria (‘D’), tetanus (‘T’), pertussis (‘P’) andHaemophilus influenzaetype b (‘Hib’), wherein: (a) the antigen for protecting against Hib is a conjugate of a Hib capsular saccharide; ...
increased stress levels (higher heart rates, higher blood pressure, and need for sedation) in the oxygen-treated group than in the control group or evidence that oxygen treatment was associated with more infections, with little difference in the highest temperature or the need for antibiotics (...
As a loading control, the membrane was probed with a primary antibody to GAPDH (MAB374 mouse monoclonal antibody, 1:300; EMD Millipore, Darmstadt, Germany) for 1 h at room temperature and subsequently probed with HRP-conjugated goat anti-mouse (sc2005, 1:5000; Santa Cruz Biotechnology, ...
They can be considered as an array of miniature thermocouple junctions connected in series as differential pairs. These differential pairs make up cold and hot junctions that are connected by alternating n-type and p-type materials. Thermopiles operate by using temperature differences to create a ...
After probe insertion, the animal was placed into an open field (21 × 21 × 30 cm). Water was provided ad libitum and room temperature maintained between 19 and 22 ºC. Samples were collected every 20 min. Three samples were taken as baseline and the neurotransmitters dopamine (DA), ...
An in house anti-AcrA antibody was used as an internal control for AcrA samples. Beads and sera were incubated at room temperature for 1 h with shaking at 500 rpm. Beads were washed with PBS 0.05% tween 20 then incubated with a 1/200 dilution of anti-human, anti-rabbit or anti-...
solution was added at room temperature and incubated for an additional 30 min in the absence of light according to the manufacturer's instructions (KGI Biology, Nanjing, China, KGA512), then the FACs Calibur system (BD Biosciences, Franklin Lakes, NJ, USA) was used for cell cycle analysis....
To monitor the translocation of the myc- GLUT4-mCherry fusion protein under a confocal microscope, the C2C12 cells expressing myc-GLUT4-mCherry were fixed in 2% paraformaldehyde for 10 min at room temperature and immunostained in a non-permeabilized condition [32]. Then, the cells were ...