Table II. Selected Normative Data for the Rata Adult weight Male 300–500 gm Female 250–300 gm Life span 2.5–3 years Body temperature 37.5°C Basal metabolism rate (400 gm rat) 35kcal/24hr Chromosome number (diploid) 42 Puberty 50 ± 10 days Gestation 21–23 days Litter size...
Obesity is a prominent risk factor for male infertility, and a high-fat diet is an important cause of obesity. Therefore, diet control can reduce body weight and regulate blood glucose and lipids, but it remains unclear whether it can improve male fertil
Frozen tissues were embedded in optimal cutting temperature (OCT) compound and cryo-sectioned at 25 μm on PEN-membrane slides and fixed in 70% ethanol for 5 min. Fixed tissue sections were rinsed twice with 1X PBS, stained with haematoxylin and eosin (H&E), and photo-recorded using ...
After 30 minutes the cells were resuspended in 1% osmium tetroxide, 0.1 mol/L sodium cacodylate, pH 7.2 for 15 minutes at room temperature and then washed twice in sodium cacodylate. This procedure prevented the formation of nonspecific aggregates while still preserving good ultrastructural morphology...
62. A mutation arising in an early embryonic cell may be present in a substantial proportion of adult cells and in multiple different tissues59. Early embryonic mutations can be detected in whole-genome sequences of highly polyclonal adult tissue samples as mutations with relatively high VAF59,60...
Membrane was blocked in Odyssey blocking buffer (LI-COR 927-40000) for 90 minutes at room temperature. Primary antibody against Erythropoietin (Abcam ab129452, 1:200) was diluted in blocking solution and left overnight at 4 °C. Following 6 × 10 minute washes in 0.1M PBS,...
It is found that the thermal history is important for aPL binding. The incubation at 37°C is more favorable for aPL binding than ambient temperature. Normal human serine (IgG-NHS) did not elicit any distinct change of NBD-PE fluorescence, which indicates it does not interact with the lipid...
above. The localisation of the biotin tracer was visualised by incubation with Streptavidin Alexa Fluor™ 546 conjugate (S11225, Thermo Scientific, UK), diluted 1:200 in PBS, for 1 hr at room temperature. Tissue sections were imaged using an LSM 780 confocal microscope (Carl Zeiss, UK)....
After 30 min of incubation 1.2 ml of H2O and 1 ml of chloroform were added, mixed and subsequently centrifuged (10 min, 1,500 r.p.m. at room temperature). The chloroform layer was transferred into a new glass tube, and was evaporated using nitrogen at 50 °C. Lipids were resolved in...
62. A mutation arising in an early embryonic cell may be present in a substantial proportion of adult cells and in multiple different tissues59. Early embryonic mutations can be detected in whole-genome sequences of highly polyclonal adult tissue samples as mutations with relatively high VAF59,60...