Room temperature (°C) 18–26 Relative humidity (%) 30–70 Ventilation (air change/h) 10 Light/dark cycle (hours) 12–14/12–10 Minimum cage floor size Housed individually (cm2) 350 Breeding with pup (cm2) 800 Group housed (cm2 adult) 250 General Lifespan (years) 2.5–3.0 Surfa...
Frozen tissues were embedded in optimal cutting temperature (OCT) compound and cryo-sectioned at 25 μm on PEN-membrane slides and fixed in 70% ethanol for 5 min. Fixed tissue sections were rinsed twice with 1X PBS, stained with haematoxylin and eosin (H&E), and photo-recorded using ...
60,61,62. A mutation arising in an early embryonic cell may be present in a substantial proportion of adult cells and in multiple different tissues59. Early embryonic mutations can be detected in whole-genome sequences of highly polyclonal adult tissue samples as mutations with relatively high...
After 30 minutes the cells were resuspended in 1% osmium tetroxide, 0.1 mol/L sodium cacodylate, pH 7.2 for 15 minutes at room temperature and then washed twice in sodium cacodylate. This procedure prevented the formation of nonspecific aggregates while still preserving good ultrastructural morphology...
Obesity is a prominent risk factor for male infertility, and a high-fat diet is an important cause of obesity. Therefore, diet control can reduce body weight and regulate blood glucose and lipids, but it remains unclear whether it can improve male fertil
Membrane was blocked in Odyssey blocking buffer (LI-COR 927-40000) for 90 minutes at room temperature. Primary antibody against Erythropoietin (Abcam ab129452, 1:200) was diluted in blocking solution and left overnight at 4 °C. Following 6 × 10 minute washes in 0.1M PBS,...
is not known. If elevated in normal cells, the magnitude of the increase, whether it is raised over the whole lifespan, the range of tissues and fraction of cells in each tissue it affects, and the impact of subsequent neoplastic change are important questions to address in elucidating the ...
Serum was obtained by collecting blood in clot activator tubes left for 30 min at room temperature before centrifugation and plasma was prepared from blood sampled in pre-chilled EDTA tubes, which were centrifuged immediately at 4 ◦C. Insulin, C-peptides, NEFA, and TG values were analyzed ...
The prefabricated propidium iodide staining solution was added at room temperature and incubated for an additional 30 min in the absence of light according to the manufacturer's instructions (KGI Biology, Nanjing, China, KGA512), then the FACs Calibur system (BD Biosciences, Franklin Lakes, NJ,...