Adult weight Male 300–500 gm Female 250–300 gm Life span 2.5–3 years Body temperature 37.5°C Basal metabolism rate (400 gm rat) 35kcal/24hr Chromosome number (diploid) 42 Puberty 50 ± 10 days Gestation 21–23 days Litter size 8–14 Birth weight 5–6gm Eyes open 10–12...
Frozen tissues were embedded in optimal cutting temperature (OCT) compound and cryo-sectioned at 25 μm on PEN-membrane slides and fixed in 70% ethanol for 5 min. Fixed tissue sections were rinsed twice with 1X PBS, stained with haematoxylin and eosin (H&E), and photo-recorded using ...
Vital River Corporation (Beijing, China) provided 7-week-old male and 9-week-old female C57BL/6 mice for this experiment, which were housed in rooms controlled in terms of temperature at 22–25 °C and 12-h light/dark cycle. In a randomization procedure, male mice were randomly placed ...
Permeability of the blood-testis-barrier to a biotin tracer was carried out as previously described49with minor modifications. Briefly, EZ-link sulfo-NHS-LC-biotin (Thermo Scientific, UK) was freshly prepared as a 10 mg/mL solution in 0.01 M MgCl2 in PBS. 10–15 uL of biotin solu...
Serum was obtained by collecting blood in clot activator tubes left for 30 min at room temperature before centrifugation and plasma was prepared from blood sampled in pre-chilled EDTA tubes, which were centrifuged immediately at 4 ◦C. Insulin, C-peptides, NEFA, and TG values were analyzed ...