必应词典为您提供long-termculture的释义,网络释义: 造血细胞长期培养;长期耕作;
Alveolar macrophages (AMs) are lung tissue-resident macrophages that can be expanded in culture, but it is unknown to what extent culture affects their in vivo identity. Here we show that mouse long-term ex vivo expanded AMs (exAMs) maintained a core AM
et al. Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids. Nat Protoc 16, 1936–1965 (2021). https://doi.org/10.1038/s41596-020-00474-1 Download citation Received09 June 2020 Accepted12 November 2020 Published10 March 2021 Issue DateApril...
long-term cultureTo study the effects of culture media and genotypes on anther culture, anthers of sixteen durum wheat genotypes were cultured on N_6 NB medium with BA and NAA and N_6 DK medium with 2,4-D and kinetin. Overall, the calli and plants obtained were more than...
Here we provide an optimized, highly versatile protocol for long-term culture of organoids derived from either normal human breast tissues or breast cancer (BC) tissues, as well as culturing conditions for a panel of 45 biobanked samples, including BC organoids covering all major disease ...
Beyond differentiation, the lack of endogenous neurotrophic factors in neuronal cultures due to the absence of glial cells can result in apoptosis and therefore it has become common practice to add growth factors to culture media. However, the use of trophic factors is potentially problematic as sig...
Mammalian spermatogenesis is an important research subject in reproductive biology.For performing the researches on goat spermatogenesis,a method was set up first time for the primary culture of testicle germ cells from cashmere goat.The primary germ cells and Sertoli cells came from pieces of the te...
This paper evaluates the success of the European Union City/Capital of Culture programme as a model for culture-led regeneration by assessing the long-term cultural impacts of Glasgow's experience in 1990. These cultural impacts, seen as distinct from economic, physical and even social impacts, ...
Long-term proliferation of mouse primordial germ cells in culture PRIMORDIAL germ cells (PGCs) are first identifiable as a population of about eight alkaline phosphatase-positive cells in the 7.0 days postcoitum mouse emb... JL Resnick,LS Bixler,L Cheng,... - 《Nature》 被引量: 2152发表: ...
In the present investigations, we have used an adenoviral vector expressing the green fluorescent protein (AdGFP) to quantify transduction of primitive HPCs and assess adenoviral-associated toxicity in long-term culture. Here we show that a cytokine cocktail protects mass populations of CD34+ cells ...