Considering that plants phenotypic plasticity is improved under stress, this research investigated this physiological event in Neoregelia johannis microplants in long-term in vitro culture. Histological and histochemical analyses were done to monitor the morphogenic potential during the propagules induction ...
2) long-term culture 长期培养 1. Objective:To investigate the generative or regenerative hair cell and studes of molecular and genetic in the inner ear,the long-term culture systems of utricular sensory epithelial cell of the rats were established. 目的:建立椭圆囊感觉上皮细胞(USEC)长期培养...
TheumbilicalcordwasprovidedbyhealthytermbirthwomaninTianjinThirdCentralHospital.Mesenchymalstem cellswereisolatedfromhumanumbilicalcordbyenzymedigestionmethod.Cellswerepassagedat80%90%confluent.Theninth passageofcellsatadensityof5×10 10 /Lwereseededin12-wellcultureplateandincubatedwithDMEMcontaininghepatocyte growth...
Here, we show that chicken early blastoderm contains cells characterised as putative avian embryonic stem (ES) cells that can be maintained in vitro for long-term culture. These cells exhibit features similar to those of murine ES cells such as typical morphology, strong reactivity toward specific...
Long-term proliferation of mouse primordial germ cells in culture PRIMORDIAL germ cells (PGCs) are first identifiable as a population of about eight alkaline phosphatase-positive cells in the 7.0 days postcoitum mouse emb... JL Resnick,LS Bixler,L Cheng,... - 《Nature》 被引量: 2152发表: ...
Here we provide an optimized, highly versatile protocol for long-term culture of organoids derived from either normal human breast tissues or breast cancer (BC) tissues, as well as culturing conditions for a panel of 45 biobanked samples, including BC organoids covering all major disease ...
Alveolar macrophages (AMs) are lung tissue-resident macrophages that can be expanded in culture, but it is unknown to what extent culture affects their in vivo identity. Here we show that mouse long-term ex vivo expanded AMs (exAMs) maintained a core AM
Here we provide an optimized, highly versatile protocol for long-term culture of organoids derived from either normal human breast tissues or breast cancer (BC) tissues, as well as culturing conditions for a panel of 45 biobanked samples, including BC organoids covering all major disease ...
Long-term culture-initiating cells (LTC-IC) are hematopoietic progenitors able to generate colony-forming unit-cells (CFU) after 5 to 8 weeks (35 to 60 days) of culture on bone marrow (BM) stroma and represent the most primitive progenitors currently detectable in vitro. We have recently rep...
goat spermatogenesis,a method was set up first time for the primary culture of testicle germ cells from cashmere goat.The primary germ cells and Sertoli cells came from pieces of the testis seminiferous tubules of the cashmere goat.The co-cultured cells were survival in vitro over three months....