生科云网址:https://www.bioincloud.tech/ 从生物体内取出组织或细胞,在体外(in vitro)模拟体内生理环境,在无菌、适当温度和一定营养条件下,对这些组织或细胞进行孵育培养,使之保持一定的结构和功能,以便于我们观察研究,这种方法就是细胞培养(cell culture)。有时细胞...
We recently reported that peripheral blood monocytes can be induced to differentiate in vitro into hepatocyte-like cells (eo-hepatocytes) following partial de differentiation, the latter of which is associated with renewed proliferative activity and the acquisition of plasticity. Prompted by the observatio...
DEF cells were cultured for 24 h, after which the cells were washed once with PBS and cultured in serum-free cell culture medium with or without CQW1 DTMUV for 36 h. Exosomes were purified from serum-free supernatants of DEF cells with or without DTMUV infection according to the manufactur...
In the present study, antioxidant activity of methanolic ex... MN Ravishankara,N Shrivastava,H Padh,... - 《Phytomedicine》 被引量: 175发表: 2002年 Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture A procedure for rapid in vitro propagation of the aromatic and...
The cell culture rea- gents including DMEM mediem and fetal bovine serum (FBS) and so on were from Gibco (AG, Switzerland). Bey- otime (Shanghai, China) provided Calcein-AM, PI and DAPI staining solutions, and Cell Counting Kit-8 (CCK- 8). The antibodies for immunostaining were from...
coli were inoculated into 20 mL Luria-Bertani (LB) medium and cultured in an incubator at 200 rpm at 37 °C. When the OD600 value of the culture reached 0.1, the bacteria were centrifuged at 10,000× g for 5 min, and collected and washed with phosphate-buffered saline (PBS, pH 7.5)...
Sex could be differentiated as early as 17 hours in both male and female worms Although complete maturation was not obtained, the copulatory bell or everted cloaca and copulatory appendages in the male and the vagina and vulva in the female show that it is possible to obtain in vitro ...
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. 实验时长4.5h实验方法双抗夹心法 规格96T ELISA Kit for Defensin Beta 2 (DEFb2) FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
[3]. Topper, R. J., Oka, T. & Vonderhaar, B. K. Techniques for studying development of normal mammary epithelial cells in organ culture. Methods Enzymol. 39, 443–454 (1975). [4]. Hardman, P., Klement, B. ...
Recently, another study using a murine tumor cell line (B16 cells) showed that cell culture in riboflavin-depleted medium leads to a decrease of 13% in the abundance of 68 flavoproteins, and one of the most affected was GCDH [13]. These reports highlight the impact of FAD availability on...