Basic cell culture protocols. Totowa, NJ: Humana Press. Vol. 946, p. 241-256.Human long-term culture initiating cell assay. Liu M,Miller CL,Eaves CJ. Methods in Molecular Biology . 2013Liu M , Miller CL , Eaves CJ . 2013 . Human long-term culture initiating cell assay . In: ...
Long-term initiating cell assayLTC-ICStromal cellsHematopoiesisThe long-term culture initiating cell (LTC-IC) assay, founded on the bone marrow long-term culture (LTC) system, measures primitive hematopoietic stem cells (termed LTC-IC) based on their capacity to......
10 normal donors with regard to their content in long-term culture-initiating cells (LTC-IC) as assessed by a limiting dilution assay and the clonogeneic capacity of these cells, in order to determine whether remission marrow cells displayed any specific defect at the primitive stem cell level...
In a long-term culture initiating cell (LTC-IC) assay, an eight-fold decrease of early progenitor cells was observed in the marrow of transplanted patients compared to normal donors, and a five-fold decrease was documented in peripheral blood. We found that the BM LTC-IC cell number ...
while the number of PB CD34+cells was the same as in normal donors. Using a methylcellulose colony assay, primary BM colony-forming cells (CFU-GM) were decreased 2.1-fold, whereas PB CFU-GM were only marginally decreased. In a long-term culture initiating cell (LTC-IC) assay, an eight...
9,30 Clinical scale-up of a gene therapy approach would be greatly facilitated by the use of such an enriched target cell population. We have demonstrated efficient gene transfer to human bone marrow progenitors of all lineages and long-term culture-initiating cells (LTC-ICs) using either ...
Assays for primitive progenitors in long-term culture CD34+ cells were cocultured over the MS5 murine bone marrow stromal cell line29 in 12.5% FCS/12.5% horse serum/IMDM supplemented with 7.9 μg/mL monothioglycerol, 10−6 mol/L hydrocortisone, 20 mmol/L glutamine and 750 μg/mL gentamyci...
Murine stromal cells counteract the loss of long-term culture-initiating cell potential induced by cytokines in CD34(+)CD38(low/neg) human bone marrow cells. Evidence has been provided recently that shows that high concentrations of cytokines can fulfill functions previously attributed to stromal cells...
AC133ϩ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/ SCID-repopulating cells and dendritic cell ... - 《Stem Cells》 被引量: 211发表: 2010年 CD34+AC133+ Cells Isolated from Cord Blood are Highly Enriched in Long-Term Culture-Initiating Cel...
Therefore, we examined whether the long-term culture-initiating cells (LTC-IC) supportive capability of HS is attributable to an oligosaccharide of defined length and protein-binding ability. Oligosaccharides of a wide range of sizes were prepared, and their capability to support human marrow LTC-...