舒桐科技在过去的2年中打造了一站式基因编辑安全性评估平台,覆盖了靶点设计、靶点筛选、GUIDE-seq脱靶检测(cellular-based assays)、AID-seq脱靶检测(biochemical assay)、脱靶验证、PEM-seq染色体重排检测、三代Long Range PCR、载体插入位点检测(升级版LM-PCR及液相杂交捕获测序)、克隆扩增分析、转录组脱靶检测、WGS...
The polymerase chain reaction (PCR) has been used extensively in the amplification of DNA fragments where there is a general size preference for products less than 1000 bp. Larger products may be synthesized at low or inefficient levels and then normally after considerable effort in reaction ...
Long-range PCR with LA Taq Direct PCR from human nail Direct PCR from meat samples Megaprimer PCR with PrimeSTAR GXL Amplifying GC-rich templates Titanium Taq for high-throughput genotyping Colony PCR in under an hour High-throughput endpoint PCR ...
demarcated by a set of oligonucleotide primers. Long-range PCR is a form of PCR optimized to facilitate the amplification of large fragments. Using the adapted long-range PCR protocol described in this chapter, we were able to generate PCR products of 6.6, 7.2, 13, and 20 kb...
本发明提供一种用于检测CYP2D6基因多态性变异的Long‑rangePCR方法及试剂盒。其方法包含:步骤1.基因组DNA提取,步骤2.LR‑PCR法特异性扩增CYP2D6基因,步骤3.扩增产物纯化,步骤4.产物文库构建。相对于多重PCR检测,该检测方法无需设计特别多对的引物,而且在PCR过程中减少非特异性条带的扩增,有效的对多态性变异条...
each 5µl (0.2mMofeach) 5µl (0.2mMofeach) Forwardprimer0.3-1µM0.3-1µM Reverseprimer0.3-1µM0.3-1µM TemplateDNA10pg–1µg1ng–1µg DMSO–2µl(4%) LongPCREnzymeMix1.25-2.5u2.5u Water,nuclease-freeto50µlto50µl 3.Gentlyvortexthesamplesandspindowntocollect drops...
2.一种用于检测CYP2D6基因多态性变异的Long‑range PCR方法, 其特征在于, 所述方法包括: 步骤1.基因组DNA提取、 步骤2.LR‑PCR法特异性扩增CYP2D6基因、 步骤3.扩增产物纯化和步骤4.产物文库构建;所述步骤1.提取待测样本的基因组DNA, 所述待测样本类型包括: 血液、组织、 唾液、 口腔拭子或毛发, 并测定...
イルミナウェビナー 2013年年6⽉月19⽇日 疾患関連遺伝⼦子の long-‐‑‒range PCR Nextera 解析 国⽴立立遺伝学研究所 ⼈人類遺伝研究部⾨門 細道⼀一善 本Webinarについて ⽐比較的⼿手軽な次世代シーケンサーの使い⽅方 ・数⼗十kb程度度の領...
Long Range PCR技术的方法学研究与初步应用
A Novel Long-Range PCR Sequencing Method for Genetic Analysis of the Entire PKD1 Gene Genetic testing of PKD1 and PKD2 is useful for the diagnosis and prognosis of autosomal dominant polycystic kidney disease; however, analysis is complicate... YC Tan,A Michaeel,J Blumenfeld,... - 《Journal ...