Long-range PCRFLGPacBioSMRTbell libraryProtocol optimizationNGSThe filaggrin (FLG) protein, encoded by theFLGgene, is an intermediate filament-associated protein that plays a crucial role in the terminal stages of human epidermal differentiation. Loss-of-function mutations in theFLGexon 3 have been ...
Long Range Land Attack Projectile (LRLAP) - Lockheed :长距离地攻击弹(LRLAP)-洛克希德 热度: 一步法实时荧光RT-PCR反应缓冲液及其反应体系和PCR方法 热度: PCR反应增强剂组合物、微滴式数字PCR反应液及其应用 热度: 相关推荐 P r o t o c o l Weo ertwoproductssuitable orlongrangePCR:Long PCR...
coli liquid lysates by a simple protocol, is used as template in PCR following a commercial protocol. The method produces library DNA sufficient for several hundred PCRs, incorporates nested primers to reduce nonspecific product formation, and enables the synthesis of linker-containing DNA fragments ...
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Long-range enhancer–promoter contacts in gene expression control. Nat. Rev. Genet. 20, 437–455 (2019). Article CAS PubMed Google Scholar Chuong, E. B., Elde, N. C. & Feschotte, C. Regulatory evolution of innate immunity through co-option of endogenous retroviruses. Science 351, ...
RACE-PCR, RT-PCR, and Long-Range PCR Oligo-dT and random-primed cDNA libraries were constructed from poly(A)+ mRNA of human total fetus, adult brain, heart, kidney, and murine fetal heart, using a Marathon cDNA amplification kit (Clontech). RACE-PCR experiments were performed on these lib...
Briefly, the PCR (50 μl) was conducted using 10 pmol of each of the two oligonucleotide primers, 100 μM of each dNTP, 3 mM MgCl2 and 1 U of BD Advantage 2 Taq polymerase (BD Biosciences) using an ABI 2720 thermal cycler (ABI), employing the following cycling protocol: one cycle ...
Although great progress had been made in genome phasing by both computational and experimental methods, long-range haplotype phasing is still technically challenging18. In SCS, because the homologous chromosomes are physically separated, we could obtain haplotype phasing information of the entire chromosome...
C) Read number and read length in two fibroblast cell line samples (one of which was sequenced in replicate) that were sequenced using both the direct-cDNA and the PCR-cDNA protocol for 48 h. P values were calculated using a two-sided t-test. Error bars: standard deviation from the ...
Long-range PCR An aliquot of the long-range PCR product was run on the Bioanalyzer DNA 12000 chip assay, which showed the presence of five fragments of different length (~480, 930, 2000, 2900 bp, and 8.8 Kb, Fig.1a supporting information). The following analysis showed that the larg...