Long-range PCR products Reduce the time and effort needed for amplifying your long targets by choosing a polymerase that can go the distance. Our DNA polymerases are optimized for amplifying long targets (up to 48 kb), including repetitive, AT-, or GC-rich sequences. Choose from theTakara ...
The polymerase chain reaction (PCR) has been used extensively in the amplification of DNA fragments where there is a general size preference for products less than 1000 bp. Larger products may be synthesized at low or inefficient levels and then normally after considerable effort in reaction ...
Example 3: Long-range PCR using human genomic DNA Methods The TPA gene region and the β-globin cluster region were each amplified from human genomic DNA usingTakaraLA TaqDNA polymerase. The reactions were set up as indicated below: ComponentVolumeFinal concentration ...
Long Range PCR技术的方法学研究与初步应用
Proofreading DNA polymerase fusions offer several advantages for long-range PCR, including faster run times and higher fidelity compared with Taq-based enzymes. However, their use so far has been limited to amplification of small to mid-range targets. In this article, we present a modified protoc...
Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus 来自 NCBI 喜欢 0 阅读量: 50 作者:R Wendy,SD Kane,JM Bullard 摘要: DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three...
2X Long Range PCR DNA Polymerase Master Mix Blue (2mM MgCl2) Cat. No.: 180601-1 Size: 1.25 mL Storage:-20 General Description Taq/Pfu DNA Polymerase Master Mix Blue -- , it is a enzyme mixture of Taq and pfu DNA polymerase, it provides more efficient amplification and higher fidelity...
pcrreactionlongrangeset距离 P r o t o c o l Weo ertwoproductssuitable orlongrangePCR:Long PCREnzymeMixandHighFidelityPCREnzymeMix. TheenzymemixescontainTaqDNApolymeraseanda thermostabilehighdelityDNApolymerasewith proo readingactivity.Duetotheintrinsic3'≥5'exonucle- aseactivityo thehighdelityDNApolyme...
Long‐range PCR 来自 ResearchGate 喜欢 0 阅读量: 128 作者: S Wilton 摘要: The polymerase chain reaction (PCR) has been used extensively in the amplification of DNA fragments where there is a general size preference for products less than 1000 bp. Larger products may be synthesized at low or...
A polymerase chain reaction (PCR)-based strat... A Butler,AS Whitehead - 《Immunogenetics》 被引量: 29发表: 1996年 Long-Range Polymerase Chain Reaction for Specific Full-Length Amplification of the Human Keratin 14 Gene and Novel Keratin 14 Mutations in Epidermolysis Bu... The present study ...