BaseSpace Sequence Hub DRAGEN Secondary Analysis Illumina Connected Analytics Emedgene Illumina Connected Insights Clarity LIMS Correlation Engine Microarray Software All Software & Informatics Products DRAGEN v4.3 now available on-premises and on-cloud Next-generation multigenome mapper, expanded ORA compressio...
自从20年前下一代测序发明,科技创新的脚步从未放慢。虽然由很多厉害的算法努力通过重叠序列(overlapping sequences)连接短的读段(reads),但由于基因组的绝对长度和复杂性使得很难生成完整序列,经常会导致许多缺失部分和错误。这推动了许多long-read sequencing策略的发展。两个最常用的商用技术就是: ...
Segmental Duplication Assembler (SDA; https://github.com/mvollger/SDA) constructs graphs in which paralogous sequence variants define the nodes and long-read sequences provide attraction and repulsion edges, enabling the partition and assembly of long reads corresponding to distinct paralogs. We ...
The PacBio and ONT sequencing are able to read full-length transcripts and allow the direct study of base modifications on both DNA and RNA molecules. Nanopore technology is able to sequence RNA directly. LRS has revealed a much more complex viral transcriptome. Among other capabilities, these t...
a protein25, causes nonsense-mediated decay (NMD)88, contains repetitive elements93, is a transmembrane protein94, contains a domain motif95, contains a signal peptide96, or has intrinsically disordered regions (IDRs)97 in silico based on the nucleotide sequence obtained by long-read sequencing. ...
PacBio incorporated the NVIDIA A100 Tensor Core GPU into its Revio system to accelerate the speed and accuracy of long‑read sequencing, while minimizing costs.
The long read sequence information is promised to enhance the quality of incomplete and inaccurate draft assemblies constructed from Next Generation Sequencing (NGS) data. Here we propose a novel hybrid assembly methodology that aims to scaffold pre-assembled contigs in an iterative manner using Pac...
Single-molecule real-time (SMRT) sequencing, a third-generation sequencing (TGS) technique recently developed by Pacific Biosciences (PacBio), allows direct sequencing of full-length, single-molecule cDNA sequences with a read length of up to 20 kb9,11,16. Using PacBio SMRT sequencing, intact...
Mind the Gap: Upgrading Genomes with Pacific Biosciences RS Long-Read Sequencing Technology. Many genomes have been sequenced to high-quality draft status using Sanger capillary electrophoresis and/or newer short-read sequence data and whole genome... Yu-Min,Lin,Chin-Jung,... - 《Plos One》 ...
generating a single long read (Fig.1). This long read is then separated into subreads, each corresponding to a single polymerase pass through the DNA molecule. The alignment of subreads eventually generates a highly accurate consensus sequence. This correction method is allowed by the stochastic ...