然而,lncRNA在大小上存在显著差异,==长度超过10 kb的lncRNA属于超长基因间RNA (very long intergenic RNAs, vlinc)和宏观lncRNA==。这些转录本具有一些区别于其他lncRNA的特征:它们的剪接性差或不剪接,在3 '端弱聚腺苷酸化,并且由特定的基因组位点产生。大多数vlincRNA都位于同一条或相反的链上的PCG(蛋白编码...
Hung T and Chang HY: Long noncoding RNA in genome regulation: Prospects and mechanisms. RNA Biol 7: 582‑585, 2010.Hung T, Chang HY: Long noncoding RNA in genome regulation: prospects and mechanisms. RNA Biol 2010, 7:582-585....
《Long Non-Coding RNA Biology》是一本由M.R.S. Rao著的专业书籍,该书于2017年由Springer出版社出版。原著中说明了可以用于交流学习,不涉及商业用途就可以,因此不存在侵权问题。 本书是关于长非编码RNA(lncRNA)的综合性教材,涵盖了lncRNA的多个方面,包括结构、功能、调节机制、疾病相关性等等。该书共分为10章...
lncRNA荧光定量PCR实验 长链非编码RNA(long noncoding RNA, lncRNA)是一类长度超过200nt的非编码RNA分子。哺乳动物基因组序列生成的转录本中约有4%~9%是 lnc RNA,而1%为编码RNA。多数lnc RNA生成与 m RNA 类似,都由RNA 聚合酶Ⅱ转录和剪切,生成 poly(A)尾。lnc RNA主要定位于细胞核中,少数分布在细胞质。
Paradigms of Long NoncodingRNAFunction in the Regulation of Gene Expression 106 4.1.1 Long NoncodingRNAsas Guides for the Epigenetic Machinery 108 4.1.2 Long Noncoding RNAInterferes with Other Noncoding RNA Functions 109 4.2 Long Noncoding RNAs are Associated with Disease Risk 109 ...
MALAT1 has previously been described as a metastasis-promoting long noncoding RNA (lncRNA). We show here, however, that targeted inactivation of the Malat1 gene in a transgenic mouse model of breast cancer, without altering the expression of its adjacent genes, promotes lung metastasis, and tha...
名词解释长非编码 RNA(long non-coding RNA,IncRNA) 答案 长非编码RNA:是一类长度为200~100000个核苷酸的RNA分子。lncRNA由RNA聚合酶Ⅱ转录生成,经剪切加工后,形成具有类似于mRNA的结构。lncRNA有poly(A)尾和启动子,但序列中不存在可读框,不编码任何蛋白质。lncRNA具有调控的多样性,可从染色质重塑、转录调控及转...
Real-time PCR gene expression and long non-coding RNA workflow: Step 1:Isolate RNA Step 2:Design and optimize primers Step 3:Reverse transcription Step 4:Amplify cDNA Step 5:Run qPCR Step 6:Analyze Isolate RNA Isolate RNA using methods that ...
Long non-coding RNAs (lncRNAs) are non-protein-coding transcripts shown to play important roles in tumourigenesis and tumour progression. Our study aimed to examine expression of the lncRNA MAGI2-AS3 in breast cancer and to explore its function in cancer cell growth. First, MAGI2-AS3 ...
长链非编码RNA(long noncoding RNA, lncRNA)指的是长度超过200nt的RNA分子。它们不编码蛋白,位于细胞核或胞质内,具有保守的二级结构,可与蛋白质、DNA和RNA相互作用,通过多种机制(如基因印记、染色质重塑、细胞周期调控、剪接调控、mRNA降解和翻译调控等)在多种层面上(如表观遗传学、转录调控及转录后调控等)调控基...