we hypothesized that ectopic expression of HOTAIRM1 or HOXA1 could induce MDSC development to suppress T cell functions. To test this hypothesis, we transfected healthy CD33+cells with GFP-HOTAIRM1 or GFP-HOXA1 constructs or control plasmid (GFP-pCDNA3.1) for 3 days and then analyzed myeloid ...
LncRNA-HOTAIRM1在肺腺癌中高表达(P0.0001).59例肺鳞癌患者,癌组织中LncRNA-HOTAIRM1相对表达量为2.288±2.222,癌旁组织中的相对表达量为1.00±1.037,与癌旁组织相比较,LncRNA-HOTAIRM1 在肺鳞癌中高表达(P0.0001).2,分析LncRNA-HOTAIRM1表达与NSCLC患者临床病理特征的关系,结果表明,在低分化NSCLC患者中,Lnc...
然后利用与lncRNA存在连接的mRNA来预测lncRNA功能。例如,拟时间相关的lncRNA HOTAIRM1在hESC的第6天和第9天高表达,其与105个mRNA基因和13个lncRNA基因连接。基于这些mRNA功能富集分析,HOTAIRM1被赋予“调节胞吐作用”,“视黄酸受体信号通路”等功能(图5C)。这与前人报道的HOTAIRM1的功能一致。 以上结果证明了基...
(sh HOTAIRM1,sh HOTAIRM1+HOTAIRM1)经尾静脉注入NOD/SCID小鼠以构建白血病动物模型,生存曲线分析小鼠总生存时间;瑞氏染色后在显微镜下进行小鼠骨髓象分析;苏木精-伊红(HE)染色观察肝脾组织中白血病细胞的浸润情况;通过免疫组织化学(IHC)染色检测Ki67增殖抗原观察白血病细胞增殖情况;western blot检测自噬关键分子LC3和...
(3)Zhang X 等发现在全反式维甲酸( ATRA) 诱导急性早幼粒细胞白血病( APL) NB4 细胞株粒系分化过程中,lncRNA HOTAIRM1 的表达显著增加 HOTAIRM1 编码在 HOXA 基因簇,是一种基因间 lncRNA,敲减 HOTAIRM1 后会显著抑制ATRA 诱导的 NB4 细胞株粒系分化,并且选择性地减弱骨髓分化基因CD11b和CD18的转录物...
HOTAIRM1 silencing and miR-148b overexpression both suppressed cell proliferation and induced apoptosis in AML cells. miR-148b was identified as a target of HOTAIRM1 in AML cells. Moreover, HOTAIRM1 knockdown inhibited proliferation and induced apoptosis in AML cells by negatively regulating miR-...
Cell Death and Differentiation (2017) 24, 212–224 Official journal of the Cell Death Differentiation Association www.nature.com/cdd OPEN The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway Zhen-Hua Chen1, Wen-...
有研究表明,LncRNA NEAT1在IBD中高表达,其可通过降低紧密连接蛋白Zonula occludens-1、Occludin水平导致肠上皮屏障完整性受损,并且还能激活M1巨噬细胞,促进肠道炎症反应的发生[24]。此外,在一项5-氨基酮戊酸(5-ALA)-光动力(PDT)治疗IBD的研究者中发现,ln...
Furthermore, lncRNA transcriptome sequencing coupled with RNA immunoprecipitation (RIP) revealed that PUM1 inhibits trophoblast invasion in PE by downregulating the expression of lncRNA HOTAIR. Moreover, PUM1 regulates HOTAIR expression via a posttranscriptional mechanism. Using RNA-protein pull-down ...