We present a Monte Carlo simulation of the bandwidth of an InAlAs avalanche photodiode with an undepleted absorber. The carrier velocities are simulated in the charge layer and the multiplication region. It is shown that the velocity overshoot effect is not as significant as simpler models have ...
These HLCs are integrated into various culture systems including 2D culture, 3D organoids or spheroids, bioprinted tissue constructs, and liver-on-a-chip (microfluidic) devices. The culture systems can then be used to model various liver diseases including HBV, HCV, malaria, NAFLD, ALD, and ...
With the Emulate human Liver-Chip, researchers can test the delivery efficiency and safety of adeno-associated virus (AAV) vectors in a human-relevant model of the liver sinusoid and get results in weeks—not months, like with animal models. With this application, it is possible to rapidly it...
LiverChip TM Scaffold Figure1.LiverChip TM scaffold. AttheheartofLiverChip TM isascaffoldwhose dimensionshavebeenengineeredtorecreatethe capillarybedstructureoftheliversinusoid. Moreonscaffolds TheLiverChip TM scaffoldishousedwithinaspecially designedplatewhichpermitsdirectperfusionofthe ...
Here we show that liver sinusoidal endothelial cell (LSEC) senescence accelerates liver sinusoid capillarization and promotes steatosis by reprogramming liver endothelial zonation and inactivating pericentral endothelium-derived C-kit, which is a type III receptor tyrosine kinase. Specifically, inhibition of...
Moreover, they indicated that some cell crosstalk occurs during the early formation of the sinusoid. Overall, these results generated a comprehensive map of a primitive liver cell lineage from the endoderm and mesoderm. The liver is structurally and metabolically zonated, which forms the basis for...
(hepatocytes, endothelial cells, stellate cells and Kupffer cells). Leeet al.44fabricated a PDMS device which mimics the liver anatomy. The device features an artificial liver sinusoid with an artificial barrier layer mimicking endothelial barrier layer. We have also integrated cell lines and primary...
Briefly, the nuclear suspension and barcoded beads were loaded on the chip for droplet encapsulation. Beads were collected for reverse transcription using the following parameters: 10 cycles at 50 °C for 2 min followed by a step at 42 °C for 2 min. The cDNA-containing beads were then ...
Nutrient- and oxygen-rich blood from the intestine flows into the sinusoid via the portal vein. After being processed by the hepatocytes, the blood enters the central vein and into the systemic circulation. Reproduced with permission from J. Daugherty. Within the liver lobule, hepatocytes are ...
Sinusoid vessels and rows of hepatocytes radiate outward to the portal triad—composed of the bile duct, portal vein, and hepatic artery. The walls are composed of liver sinusoidal endothelial cells (LSECs) with adherent and macrophagic Kupffer cells populating the lumen. On the periphery are ...